Figure 10
A-D, photographic series of a single embryo from the 5% glutaraldehyde experimental group representing general workflow repeated for all individuals in sample. A, freshly harvested, unfixed specimen representing initial photographs overlain with landmarking scheme: red fan samples midbrain, green fan samples left telencephalon. Wireframe outline of landmarks in blue. B, same embryo fixed with 4% formaldehyde + 5% glutaraldehyde. C, 3D μCT scan. D, final digital photograph of series taken after μCT scanning. E-G, imaging series of an individual representing workflow for individuals from Bouin's experimental group. E, Bouin's fixation. F, μCT scan. G, final photo of same specimen post μCT scan. Photographic series representing individuals of 4% formaldehyde + 1% glutaraldehyde experimental group not shown. A, B, D, E, and G at same magnification. H5%, harvested embryo of 5% glutaraldehyde experimental group. F5%, fixed embryo of 5% glutaraldehyde experimental group. PS5%, post-scanned embryo of 5% glutaraldehyde experimental group. FB, fixed embryo of Bouin's experimental group. PSB, post-scanned embryo of Bouin's experimental group. All embryos are shown in left lateral view.