Figure 6
From: Generation and characterization of a Tet-On (rtTA-M2) transgenic rat
Evaluation of EGFP expression in tissues from ROSA-rtTA-M2 transgenic rat. Lenti-TetO7/CMV-EGFP was injected into the testis, hind leg skeletal muscle or kidney of ROSA-rtTA-M2 rats without (A, C, E and G) or with (B, D, F and H) Dox in their drinking water (0.5 mg/ml). A-B. Immunohistochemical analysis of EGFP expression in histological section of testicular seminiferous tubule. Counterstain: DAPI. C-D. Immunohistochemical analysis of EGFP expression in whole mount of testicular seminiferous tubule. E-F. Immunohistochemical analysis of EGFP expression in skeletal muscle. G-H. Immunohistochemical analysis of EGFP expression in Kidney. Counterstain: hematoxylin. Bar = 50 μm (all panels).