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Figure 2 | BMC Developmental Biology

Figure 2

From: BMP-2 functions independently of SHH signaling and triggers cell condensation and apoptosis in regenerating axolotl limbs

Figure 2

Expression of BMP-2 (A-C) and SOX-9 (D-F) detected by whole mount in situ hybridisation in developing limbs treated 14 days with cyclopamine. Expression of BMP-2 (G-I) and SOX-9 (J-L) in developing limbs treated 21 days with cyclopamine. Left column (A, D, G, J, M) shows control animals (0.4 μL EtOH/mL), middle column (B, E, H, K, N) shows the animals treated with 1 μg/mL of cyclopamine and right column (C, F, I, L, O) shows the animals treated with 2 μg/mL of cyclopamine. All pictures show a dorsal view of the limbs with posterior side on top. Arrowheads in C, H and K indicate the expression of BMP-2 on both sides of the regenerating digits and the expression of SOX-9 in the middle of the regenerating digits. The last row (M-O) shows the cartilage staining (Victoria blue) of the skeletal elements at the end of cyclopamine treatments (21 days).

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BMC Developmental Biology

ISSN: 1471-213X

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