Figure 8
From: Activated Ras alters lens and corneal development through induction of distinct downstream targets
Cell cycle targets in the lens and cornea. In situ hybridizations (A-J, M, N) and immunohistochemistry (K, L) were performed on nontransgenic (NT) and Pax6-Ras transgenic E13.5 embryos to detect expression of cyclin D1 (Ccnd1) (A, B), cyclin D2 (Ccnd2) (C, D), cyclin B1 (CcnB1) (E, F) and cdk inhibitors p21Cip1 (G, H), p57Kip2 (I-L) and p27Kip1 (M, N). For in situ hybridizations (A-J, M, N), dark-field images were overlaid on respective bright-field images and silver grains were colored red. For immunohistochemistry (K, L), antigen antibody complexes are in red and nuclei are stained blue with DAPI. Discontinuities in the corneal epithelium (B, C, D, F, G, H, I, asterisks) are sectioning artifacts. Cyclins D1 (B), D2 (D) and B1 (F) were upregulated in the Ras transgenic corneas. p57Kip2 (J, L) and p27Kip1 (N) were upregulated in the Ras transgenic lens epithelial cells but not corneas. p21Cip1 was upregulated in the Ras transgenic lenses and corneas. Abbreviations; C, corneal epithelium; Cj, conjunctival epithelium; le, lens epithelium; lf, lens fibers; nr, neural retina. The scale bar (G) represents 100 μm in panels A-J, M, N and 50 μm in panels K, L.