Figure 2
From: Activated Ras alters lens and corneal development through induction of distinct downstream targets
Transgene expression. In situ hybridizations were performed on ocular sections of nontransgenic (A, C, E) and Pax6-Ras transgenic (B, D, F) embryos using an 35S-labeled SV40 riboprobe. Dark-field images were overlaid on respective bright-field images and silver grains were pseudocolored red. Ras transgene expression was seen in the lens, cornea and conjunctival epithelial cells at E11.5 and E13.5 (B, D) and in some retinal neuroblasts at E13.5 and E16.5 (D, F). Discontinuity in the corneal epithelium in panel B (asterisk) is due to a sectioning artifact. Abbreviations; C, corneal epithelium; Cj, conjunctival epithelium; le, lens epithelium; lf, lens fibers; nr, neural retina. The scale bar (E) represents 100 μm in panels A-D and 250 μm in panels E, F.