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Figure 2 | BMC Developmental Biology

Figure 2

From: Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish

Figure 2

Fluorescence activated cell sorting (FACS) of dissociated cldnb:gfp tails. (A) Plot showing the P1 gate used to sort cells from tails of transgenic (cldnb:gfp) embryos, according to cell size (forward scatter) vs. granularity (side scatter). (B) Plot showing GFP fluorescence intensity of cells vs. Phycoerythrin (PE-A). P2 and P3 demarcate cells sorted as GFP- and GPP+, respectively. (C) Quantitative RT-PCR analysis (qRT-PCR) was performed of RNA derived from GFP+ and GFP- cells from tails of 36 hpf embryos. Real time PCR ratio was determined by normalization to β-actin (equal to 1, dotted line).

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