Table 1 Medfly homologues of genes involved in sex-determination and cellularization and their function and embryonic expression in D. melanogaster

CcZelda

(CcZld)

FG073766

Zelda(zld)

CG12701

138

4E-43

54

66

zinc-finger transcription factor involved in MTZ process and neuronal development

maternal origin

Ccsisterless A

(CcsisA)

FG075887

sisterless-a (sisA)

CG1641

180

3E-13

26

49

transcription factor forming the primary signal of the sex-determination cascade (XSEs) and involved in endoderm development

expressed in female zygote at the end of nuclear cycle 8 (1:12 h)

Ccdeadpan

(Ccdpn)

FG076265

deadpan (dpn)

CG8704

48

1E-10

75

83

transcription factor forming the primary signal of the sex-determination cascade (autosomal) and involved in dendrite morphogenesis

expressed in the zygote starting from nuclear cycle 12 (2 h)

Ccgroucho

(Ccgro)

FG077761

groucho (gro)

CG8384

217

6E-07

97

98

corepressor of transcription forming the primary signal of the sex-determination cascade (autosomal) and involved in dendrite morphogenesis

maternal origin, gro transcripts are detectable until stage 11 after complete gastrulation (5:20 h)

CcSex-lethal

(CcSxl)

AF026145

Sex lethal (Sxl)

CG18350

346

5E-113

66

73

RNA-binding protein, member of the sex-determination molecular cascade

expressed in the zygote starting from nuclear cycle 12-13 (2 h)

Cctransformer

(Cctra)

AF434936

AF434937

AF434938

transformer (tra)

CG16724

11

4E-03

72

90

RNA-binding protein, member of the sex-determination molecular cascade

maternal origin; an increased amount of tra transcripts is detectable 2-3 h after oviposition

Cctransformer2

(Cctra2)

EU437408

transformer2 (tra2)

CG10128

166

2E-42

56

72

RNA-binding protein, member of the sex-determination molecular cascade

maternal origin. No change in tra2 transcript amount is detectable after 2-3 h and in chromosome 2R deleted embryos

Ccdoublesex

(Ccdsx)

AF434935

AF435087

doublesex

CG11094

336a 248b

2E-55 1E-56

68 85

77 91

zinc-finger transcription factor involved in sex differentiation process

dsx transcripts are first detectable during stage 11 (9:20 h) in the fat bodies and gonad primordia

Ccsans-fille

(Ccsnf)

FG082648

sans fille (snf)

CG4528

80

2E-39

97

98

U1 snRNA binding protein involved in sex-determination and oogenesis

the SNF protein localizes to the nucleus in all tissues during development

Ccfemale-lethal d

(Ccfl(2)d)

FG070226

FG073068

FG071876

female lethal d (fl(2)d

CG6315

493

3E-91

47

53

RNA splicing factor involved in sex-determination and female-germline sex-determination

maternal origin

Ccintersex

(Ccix)

FG072637

intersex (ix)

CG13201

142

2E-54

71

85

transcription factor binding protein involved in sex-differentiation process

maternal origin; an increased amount of ix transcripts is detectable 2-3 h after oviposition

CcRho1

(CcRho1)

FG083938

Rho1 (Rho1)

CG8416

178

5E-101

100

100

protein kinase coupled with GTPase activity involved in cellular-blastoderm formation

maternal origin

Ccspaghetti-squash

(Ccsqh)

FG077819

spaghetti squash (sqh)

CG3595

153

1E-83

96

98

myosin heavy chain binding protein coupled with ATPase activity involved in cellularization and ovarian follicle cell development

maternal origin

Ccslow-as-molasses

(Ccslam)

FG068639

FJ460697

slow as molasses (slam)

CG9506

836

4E-98

32

48

protein of unknown function involved in cellularization

slam expression rapidly increases during cycle 11 (1:45 h) and peaks during cycle 14 (2:30 h)

Ccserendipity-α

(Ccsry-α)

FG072156

FJ460703

CG8247

629

3E-91

33

55

Similar to serendipity-α gene product, involved in cellularization

maternal origin