Figure 9

Shh expands the roof plate, but does not prevent formation of the hem in E10.5 injected telencephalon. E10.5 mouse telencephalon was injected with wtShh expressing retrovirus, and harvested at E12.5. Coronal sections were stained for virally infected cells using an alkaline phosphatase substrate at different rostral caudal positions (A, D, G). RNA in situ hybridization analysis was performed on adjacent sections; sections were probed using the following: Lhx5 (B), Wnt3A (C, F, I) and rTTR (E, H). n = 3. Uninjected littermate controls were identical to those shown in Figures 6, 7 and 8. Scale bar in A represents 130 μm and all panels in this figure are photographed at the same magnification (5×). R, Rostral, C, Caudal.