Skip to main content
Figure 4 | BMC Developmental Biology

Figure 4

From: Balanced Shh signaling is required for proper formation and maintenance of dorsal telencephalic midline structures

Figure 4

Shh activation prevents cortical hem and choroid plexus formation. E9.5 mouse brains were infected with wtShh, ShhN, and pCLE (alkaline phosphatase alone) expressing viruses, or uninfected littermate control (control), and harvested at E12.5. Alkaline phosphatase visualization of viral infections (A, B, F, G, K, L, P, Q) and adjacent section RNA in situ hybridization analysis of the dorsal midline of E12.5 virally infected brains, Wnt3a (cortical hem): C, H, M, R. rTTR (choroid plexus): D, I, N, S, Msx1 (cortical hem + plexus): E, J, O, T. n = 3. Scale bar in A represents 1 mm and panels A, F, K and P are photographed at the same magnification (1x). Scale bar in B represents 65 μm and panels B-E, G-J, L-O and Q-T are photographed at the same magnification (10×).

Back to article page