Figure 3

Shh activation shifts cortical gene expression laterally. E9.5 mouse brains were infected with wtShh, ShhN or pCLE (alkaline phosphatase alone) expressing viruses, and harvested at E12.5. Alkaline phosphatase visualization of viral infections (A, B, E, F, I, J) and adjacent section RNA in situ hybridization analysis of E12.5 virally infected brains, Emx-1: C, G, K, Pax-6: D, H, L (arrows indicate shifted borders of Emx-1 and Pax-6 expression). Control pCLE (A-D), wtShh (E-H), ShhN [I-L]. n = 3. All panels in this figure were photographed at the same magnification (5x). Scale bar in A represents 45 μm and panels A, E and I are shown at the same magnification. Scale bar in B represents 130 μm and panels B-D, F-H, and J-L are shown at the same magnification.