Figure 2

Shh activation ventralizes the dorsal lateral telencephalon, but not dorsal midline. E9.5 mouse brains were infected with wtShh, ShhN or pCLE (alkaline phosphatase alone) expressing viruses, and harvested at E12.5. Alkaline phosphatase visualization of viral infections (A, B, F, G, K, L, P) and adjacent section RNA in situ hybridization analysis of the dorsal midline and lateral E12.5 telencephalon of virally infected brains: Dlx-2 (C, H, M, Q), Dlx-5 (D, I, N, R), Evf _c (E, J, O, S). n = 3. Arrows indicate ventral gene expression in the cortex; asterisks identify the dorsal midline. Scale bar in A represents 1 mm and panels A, F and K are photographed at the same magnification (1x). Scale bar in B represents 65 μm and panels B-E, G-J, and L-S are photographed at the same magnification (10×).