Figure 6
In situ hybridization. Expression of mortalin in gut tissues on day 2 after evisceration. (A) Patchy in situ hybridization signal in the mesothelium of the anterior region of the cloaca. The inset shows a detailed view of the mesothelium corresponding to the boxed area on the main image. (B) Low-magnification view of the posterior mesentery. (C) A higher magnification of the free distal edge of the posterior mesentery - boxed area in (B) - showing strong hybridization signal in some cells of the mesothelium. (D) Ventral (anti-mesenterial) region of the esophageal stump showing very weak and restricted in situ hybridization signal (arrow) in the mesothelium. (E) Low-magnification view of the anterior mesentery. (F) Magnified view of the boxed area in (E) showing strongly labeled cells in the mesothelium of the free distal edge of the mesentery. de - digestive (luminal) epithelium; ct - connective tissue layer; m - mesothelium. Scale bars = 100 μm in (A), (B), (D), and (E); 25 μm in (A inset) and (C); 50 μm in (F).