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Figure 4 | BMC Developmental Biology

Figure 4

From: Visceral regeneration in a sea cucumber involves extensive expression of survivin and mortalin homologs in the mesothelium

Figure 4

In situ hybridization. Expression of survivin at early stages of gut regeneration (days 2 - 6 after evisceration). (A) Digestive (luminal) epithelium of the cloaca on day 2. (B) Free distal edge of the posterior mesentery on day 2. The inset shows a higher magnification view of the boxed area. (C) The stump of the esophagus on day 2. (D) Wall of the cloaca on day 6. (E) A low-magnification view of the posterior regenerate on day 6. (F) Higher magnification of the boxed area on (E) showing a furrow (arrowhead) of the coelomic epithelium. (G) Esophageal stump on day 6. The inset shows a lower magnification view of the cross-section of the stump. (H) Free distal margin of the anterior mesentery on day 6. de - digestive (luminal) epithelium; dm - dorsal mesentery; ct - connective tissue layer; l - lumen of the gut; m - mesothelium; vm - ventral mesentery. Scale bars = 50 μm in (A), (B inset), (F) and (G); 100 μm in (B), (G inset), and (H); 200 μm in (C) - (E).

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