Figure 3

In situ hybridization. Expression of survivin (A and B) and mortalin (C - E) in the tissues of the digestive tube in non-eviscerated animals. (A) and (B) survivin expression in the scattered cells of the luminal epithelium in the esophagus (A) and the second descending intestine (B). (C) mortalin transcripts widely expressed in the apical region of the mesothelium in the esophagus. (D) Asymmetric distribution of mortalin transcripts in the distal region of the mesentery attached to the second descending intestine. (E) mortalin-expressing cell in the luminal epithelium of the cloaca. Arrows indicate rare cells showing in situ hybridization signal. de - digestive (luminal) epithelium; ct - connective tissue layer; m - mesothelium; vm - ventral mesentery. Scale bars = 50 μm in (A) - (C); 100 μm in (D); 200 μm in (E).