Figure 12
Representative micrographs of the distribution of TUNEL-positive cells during the late phase (days 14 and 21) of visceral regeneration. (A) and (B) The anterior and posterior regenerates, respectively on day 14. (C) and (D) The newly regenerated posterior region of the esophagus and the second descending intestine on day 21 after evisceration. Insets show higher magnification views of the gut wall. de - digestive (luminal) epithelium; ct - connective tissue layer; l - gut lumen; m - mesothelium; vm - visceral mesentery. TUNEL-positive cells are green; nuclei were stained with DAPI and are shown in blue. Scale bars = 200 μm in (A) - (D); 50 μm in all insets.