Figure 11

Representative micrographs of the distribution of TUNEL-positive cells (green) in the normal gut and in the early regenerates (days 3 - 7). (A) Wall of the esophagus in a non-eviscerated animal. (B) Wall of the second descending intestine in a non-eviscerated animal. (C) and (D) The anterior and posterior regenerates, respectively, on day 3. (E) and (F) General view of the anterior and posterior regenerates, respectively, on day 7. (E') and (F') Higher magnification of the boxed areas on (E) and (F), respectively. de - digestive (luminal) epithelium; ct - connective tissue layer; m - mesothelium. TUNEL-positive cells are green; nuclei were stained with DAPI and are shown in blue. Scale bars = 50 μm in (A), (E'), and (F'); 100 μm in (B) - (D); 200 μm in (E) and (F).