Figure 6
From: β-catenin/cyclin D1 mediated development of suture mesenchyme in calvarial morphogenesis
Cyclin D1 deficiency impairs calvarial development during embryogenesis. Immunostaining analysis examines the expression of Runx2 (A, B), Osterix (OSX, C, D) and Osteopontin (OP, E, F) in cyclin D1+/+ (A, C, E) and cyclin D1-/- (B, D, F) mice. Von Kossa staining reveals that the deletion of cyclin D1 results in the absence of bone mineralization at the calvaria (Figure 6G, H). The broken lines indicate the positively stained domains. Scale bar, 0.5 mm (A-H).