Ptrg is a transmembrane protein. (A) The constructs expressing various Prtg-EGFP fusion proteins are represented by a schematic drawing. The circled numbers refer to the amino acids position from methionine (M) at the initiation site of protein translation. (B) The recombinant Prtg-EGFP fusion protein is detected along the cell surface (Prtg-full), whereas the SP-deleted Prtg-EGFP fusion protein is present in pools in the cytoplasm of cells transfected with Ptrg-ΔSP1 or Prtg-ΔSP2. (C) Immunofluorescent signals for cadherin (red) overlap with the localization of the Prtg-EGFP fusion proteins in the transfected cells. The nuclei are counterstained by DAPI.