Multiple phenotypic changes in mice after knockout of the B3gnt5gene, encoding Lc3 synthase—a key enzyme in lacto-neolacto ganglioside synthesis

Table 3 Survival rate and reproductive effects of Lc3 synthase knockout: Heterozygote breeding analysis in the further study*^†

Outcome of Breeding	Conventional Knockout	Cre-loxP Knockout
Number of delivered offspring	96	16
Number of surviving mice (survival rate)	41 (43%)^§	9 (56%)^†
Number of breeding pairs	19	13
Breeding time (months)	5	5
Number of litters	26	10
Average litter #/breeding pair	1.4	1.3
Average # of pups/litter (av # surviving/litter)	3.7 (1.6)	1.6 (0.9)

*The breeding was carried out with a heterozygote-heterozygote base. The cages were observed periodically, and the parameters shown in column 1 were recorded. Only the bodies seen dead were counted as dead mice in litter size determination.
^†About 30% of the females died during delivery for both models.
^§The percentage was compared to the theoretical number 100%. P < 0.0001 by binomial test.

ISSN: 1471-213X