Figure 9

Anti-IgG response against 3'-isoLM1 and 3',6'-isoLD1. Four B3gnt5knockout mice, each shown as a separate curve, were immunized with purified gangliosides 3'-isoLM1 and 3',6'-isoLD1 that had been conjugated to Salmonella minnesota as a carrier and adjuvant. Sera from the immunized mice were tested by ELISA for reactivity against the purified gangliosides (3'-isoLM1 and 3',6'-isoLD1) as optical density readings at 492 nm (OD 492 nm).