Figure 3

B3gnt5 gene knockout, Cre-loxP method. Cre-loxP method for targeted disruption of B3gnt5. For the Cre-loxP model, two primer pairs were used also. The exon 4 primer pair was the same as that used for conventional knockout (Figure 2). Results were analyzed as for the conventional knockout method. Another primer set detected the inserted loxP site. Both homozygous (-/-) and heterozygous (+/-) mice carried a positive 2.1-kb band, which resulted from exon 4 deletion by Cre recombinase. A 4.3-kb band indicated when the targeted portion was not deleted by Cre recombinase. No band appeared in this PCR analysis for the wild-type mice (+/+). The PCR products were sequenced to further confirm the homogeneity.