Figure 6

Dermal condensates in Prx1-Cre; Itβ1^flox/flox mice appear normal and contain primary cilia. A: Immunofluoresence for loricrin (green) in P1 control and conditional knockout mice, showing similar staining of the statrum granulosum. B: Immunofluoresence for keratin 10 (green) in P1 control and conditional knockout mce, showing similar staining of the straturm spinosum Itα6 staining in A-B shows normal basal cell layers. C: Immunofluoresence for Itβ4 (red) and p75 (green), showing normal levels of Itβ4 staining in the epithlelia. D: Immunofluoresence for p75 (green) in P1 control and Prx1-Cre; Itβ1^flox/flox mice showing similar dermal papilla expression patterns. A-D: Scale bars indicate 20 μm E: Number of dermal papilla cells in condensates of Stage 1 and 2 hair follicles of P1 control and Prx1-Cre; Itβ1^flox/flox mice. F: Confocal z-stacks of immunofluoresence for primary cilia and dermal papilla in Stage 1 (top panels) and Stage 2 (bottom panels) hair follicles of P1 control and Prx1-Cre; Itβ1^flox/flox mice. Adenylyl cyclase III (green) marks the primary cilium shaft, syndecan I (red) indicates dermal papilla cells and Hoechst dye (blue) indicates cell nuclei. Scale bars indicate 10 μm G: Percent dermal papilla cells with a primary cilium in Stage 1 and 2 hair follicles of P1 control and conditional knockout mice. All dotted lines indicate the epidermal/dermal border. Abbreviations: Ctrl, control; Itβ1 KO, dermal Itβ1 knockout; DP, dermal papilla; HF, hair follicle.