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Figure 5 | BMC Developmental Biology

Figure 5

From: Apolipoprotein C-II and lipoprotein lipase show a temporal and geographic correlation with surfactant lipid synthesis in preparation for birth

Figure 5

Distribution of apolipoprotein C-II mRNA and protein in the perinatal mouse lung. Mouse tissue sections are from saccular stage (A, B, H, GD 19.5; C, G, PN 0; I, J, PN 1; K, PN 2; L, PN 3; D to F, M, N, PN 5) or alveolar stage (O, PN 10). In situ hybridization (above the yellow line) (A to F) was performed with apoC-II anti-sense (A, C to E) and sense (B, F) probes. The major site of apoC-II mRNA synthesis (positive signal, blue) changed after birth (compare A to C). Positive signals were found in newly-formed septa (D, E) and macrophages (E) on PN 5. Immunohistochemistry (below the yellow line) (G-O) was performed using an anti-apoC-II polyclonal antibody (G to I, K to M, O) or goat IgG as negative control (J, N). Positive signals (red) were found in secretory granule-like structures in distal epithelial cells on GD 19.5 (H) and PN0 (G) but not in later timepoints. On PN 3, positive secretory granules were also found in epithelial cells of the respiratory bronchioles near the basal membrane, close to the mesenchyme (L). Macrophages were positive on PN 5 and 10 while capillaries were positive on PN 10 in one third of the analyzed subjects (O and data not shown). Scale bars, 50 μm (A to D, F, H, K, L, M, N) or 20 μm (E, G, I, J, L, O). BV, blood vessel; C, capillary; D, distal epithelium; MA, macrophage; P, proximal epithelium; RB, respiratory bronchiole; S, septa; V, vein.

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