Figure 2

Distribution of lipoprotein lipase mRNA and protein in the mouse fetal lung. Mouse tissue sections are from pseudoglandular (GD 15.5, A, B, G, H) or late canalicular (GD 17.5, C to F, I to L) stages. In situ hybridization (above the yellow line) (A to F) was performed with LPL anti-sense (A, C to E) and sense (B, F) probes. The site of LPL mRNA synthesis (positive signal, blue) changed according to gestation time. Immunohistochemistry (below the yellow line) (G-L) was performed using an anti-LPL polyclonal antibody (G, I to K) or goat IgG as negative control (H, L). Positive signals (red) were mainly found on capillaries from GD 15.5 to 17.5. Scale bars, 50 μm (A to C, E to I, K, L) or 20 μm (D, J). A, artery; C, capillary; D, distal epithelium; EC, endothelial cell; M, mesenchyme; P, proximal epithelium; SM, smooth muscle.