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Table 2 Flanking genomic sequences of Tol2 transposon insertions.

From: Optogenetic in vivocell manipulation in KillerRed-expressing zebrafish transgenics

Insertion name

Flanking sequence reads from 5' or 3' transposon ends

KR1

Insertion of a concatemer of Tol2 -containing plasmid

KR2*

5:cctACAGCAGGGCCTTCACGTCACCTCTGATACCTCGCACGGTAGACCTCATTCCAGGAAAGACCAAGAGAGCGAACAGCTGCCCTCCTCCTTAAATCCCTAAGCCAAGTGCAGTCATACCTTCATATGCCCCCTTTGGACCAACACATTCAACCTTCCACCCACTCATGAAGGAATGGCGAAAGAGGGGTGAACGGGGATATCATGTAAAAATGCTGGAAGAGAATGATGTTATATCACGAAAATAAAAACGGCTGCAGAGAGGAAGGAAAAAGAAAAATTGGGGGGAAA

 

3:aggaaaagAGCATAAACCTGCTTTAGACAGCTGTCGTTCGCCGGATCAGCTACTCAAGAGGCTGTCGTCTATTTGTTGGGCGCTAGATGTGAGCTGATCAATGGGAGACCCCTGCTGGTGTGTTTATGCAACTGCAGTGGCTTTGAAGAACTTAAGTGATCCAATGTACAATGTAGAGTGTGTTGTTGAATGGATGATATATATTTCAGTGCCTTTATTTATCAGGGGTCACCACAGTGGAATGAACCGCTAAATTATCCAGCATAGGTTTTTAC

KR4

5: catggttt TACACAGCTGATGGCCCTTCCAGCTGCAACCCAGTACGGGGGAAACACCTATACACTCATTCACACACACACACACACACACAC

KR11

3a:attaatttTTTCATTCATTTGGTTCAATTTGCCAAAATATTATTAAAATGTTACAAATTGCTTCCAAACACATCTACAACTAGCCTAAAAGGTTGATCACAGGACAAATAAGTCATAAATAGAACATAAGAAGGAGAAAATTATATTTCAATGGTTACCTGCTCTTTTGTCTATGAAGGTATTGTTGTCTCTTTGCTCAACTCACCTCTTTATGTCTTCAAATGACAGGGGTTCATTCACGGTACACTGACAGTCACATATAATCTTAACCAATGCACAGTCTAAGCTGATAGGAGCCATGATCGTTCGTTCATCACGTGACAGAATGACTTAAACCCAAGGACTCGAGAGATGAGTGGTTCAATTCTTTTTCCGGCTCGAAACGCGTATGATTGGCCCGTGATGAACGAGTGACTCAGACCCGATAGAGGACTCTAGAGGTGAGCGGATCAATTCTTTTTCCAGCTCTAAACGCATATGATTGGCTTCTGCCAATGCGATGAAGATTTGAAATTAACGATACTACCTGCACAAATGTGCGC

 

3b:gttttgcaGGCAACACTTGCTGAAAGCGGGGATCCGACAGAAGCGGAATTATGACTTGAAAACGAAAGGGCGACACTTCACAGCGGGAGAGAGGGTGTGGGTGTACAGCCCACAGAGGAAGAAGGGCAGGTGCCCTAAACTTGATGCACAGTGGGTGGGGCCTTGCATTGTACTGGAGAGAATTGGGGAAGTTGTTTACCGTGTGCAGATGCCACCAAGAGGCAGGAAAGTAGCGGTGCACAGGGATAGACTGGCTCCGTACAGAGGGTGTGCTTCAGCAGATACAGTGCTGGCCTCTCCTACAGTTATGCCTGTTCTGGAGAATGAGGCGGGACACGGGTTGGGTAGTTCTAATACTATAGATGTCCTCCTTGAAACTACACCAGTTCCTGAGCTACAAAGTTCACATTCTGGTGGCCCTGTTGCAGATAGGGGCTCACCTCGGTCTCAGAGAGTCAAGAGGCCACCAAGACGTCTCCAGGACTATGTTTGTTCCCTCGAGG

KR15

3a:gttggtcaAATATTTGTTAGCTAGCCTATCATTAAAAAGCAAAGTTATTCTGCAATCAGGTTATGTGCTCCATCAGGCACAATCTTAATAGTATCTTGTCGTGTGCTATGCCTAAGTTTTCTTTGTCTTGTAGTGTGTGCACGTTTAAGGTTTGTGTGCACTGTAACCAGATTTCATTTTCGGTTGGGATTTTAAAACTCCTGCAGCAAGTTAGGAGAAAATGAAAGCACATTAAAATGAGGAAAAATCAAATGCTTATGACAATCTTTAACATGACAAATTAAAAATGTAGCAAACAAATGACGGGTTTGTGACCCAATTACAGTGGAGCATTAAAGAAACCCTTATTTCCCACAGAACAAAAAAAATCATCTATTCATGGGCAGGAACAAAAACATACACTTGTATCTTTCTTGTATCTTCATATTATGTGCAATATTTACATTTCTAGAGAAGCCTGTTTTAAACTGTTTTGATTATTACTGACATTTATCATTCATTGCGCTGGTCACTTTAAATGAGCCAGGGCAGGCTTTACAAGCTCTTTGCAGCACTGACCTTGTTCCCAGATCACTTCGGAAGAATGAACTTGGTTGGATGAATGA

 

3b:ctaatcagTACCTCTCACTAGGGGGAGTGGGCTAAGTTTTCACATATGACATCATCGCTCTGTCTCCGACGGGTTGCTAATTCTACTACGGTGAGGGTTTGGCTTATGAATATTAAGTACGTAACGATGGTCTTCCTTAGAGGTAGCTGATTGGCTGCAAAGCGAACGTTAGTAAGCGGGATAAGGTGATGCGGTTGATTTCTTGATTAAAGGGACAGTCACATTTTTACATGCATAAACAAAGATGCATGGCTTCAATCCTAAATTTTCGACATTTTCAGATTGAAGTTGACGCTCAGCTCAAATCTTACAACAAAACAACAAAAATGTGTCCGAGTTTTGGTACTATATGAAGAACTGTCGCTGTTGATTCTGATTTGAGTAAGATAGCTTTTCTGTAATGCTCTTAATTTATTTATTTTCATACACTTTTGTTAAATTGTGATTTACTTACCCCCTTCTTTGTTTTGTTAGTCTTTCTCTCTCTG

KR19

5a:gcacctcaCTGTACAGTAGGTGACATGACAATGTAAACAAAGAACTCCGAGATGAGCAGATAAACAAACCGTATCAGTCATTCAAACAAGCCTTATACTAAATGCTTAAATCGTGTTTGCTTTTTGAAAAGAGCCACTTACTTTTTGTCCAGGTAACTTAAGGTGGAAGATGTTACATTCCGTGGCACACAACTGTCCGGTACGAACAAAAACGGTCACCAAAGGCACATACACGCGCCAATGTCCATCTGTTCGTTCTTTCTCAGTTACCAAAGTTTCCAGTCGCCGCAGTTTATACAAAAGTACAGGCTAGCGTTCATAAGCCGGGTACTCTTTGAGAGAAAGAAGCTACGCAAAGCTCAAACAAACTTTCCCATTTCCAATCGCACTGTCATCCTTCACCATATCACGAGGGGAAAAATCCGTTAATATTCCAAAGTCAAACGCGCGCTCCCTCTCTGTACAAGTCCAGTCAAGTGTTTGCAGGTGTCTAGTGTGCTGTGTGCTGTACCTCGCCCTCTCTCCACTACCTCCCTATTAAAACCAGCCAGCCGTGTCAGTGTTAACCAGCTCCCTGCCAGCGCACATGCAACTGCG

 

3b:gctgtggcGATCACAGATTAATAAAGGGACTAACCGAAAAGAAAATGAATGAATAACGCTGGATCTAACTTCGTTAACACACAAAAAACAGCTTGTTTTGATTATTGGTTTTGTTTTATTTTGCTTTTTGTCTGTTTTTTTTAGAATTTTATTTAAATTTAGGCAAAAATTTTCCCAGTGATGGGTTGCAGTTGGAAGGCCATCCGCTGCATAAAACATATGCTGGATAAGTTGGCAATTCATTCTGCTGTGGCGACCCCAGATTAATAAAGGGAATAAGCTGGAAAAAAACTAAAGAAAAATTGTTAGCTAAAATAGTCCACCCTGCCATCTAGGACTTTCTGTTTCTGTTTTTTCTTAAAGTTGTAGCATACCGCATGTAATCCATTATATTCAATGCTAATTTTTTTATGCTAAATGTAATTTTGAACAAAGCAATTGCAAACAAAAATGTATCACATTAATCCATGT

  1. To obtain the genomic location of Tol2 insertions, the flanking sequence reads were Blast analyzed against the zebrafish genome sequence database (Zv8) http://www.ensembl.org. In some cases, the flanking sequence reads were Blast analyzed against the unfinished, high-throughput genomic sequence (htgs) database or trace archive at NCBI http://www.ncbi.nlm.nih.gov/projects/genome/seq/BlastGen/BlastGen.cgi?pid=9557. Sequences flanking Tol2 ends (not listed) are highlighted in bold.