Figure 5

Depletion of cdt-2 causes a similar endocytotic defect than observed in a loss of function allele of sem-5. We analysed the uptake of VIT-2, yolk, using a vit-2::gfp expressing strain (A) and observed accumulation in body cavities in cdt-2(RNAi) (B) and in sem-5 loss of function (C). Arrows point to sites that accumulated VIT-2::GFP. We verified that the receptor, RME-2, is properly expressed using an rme-2::gfp expressing strain. No difference in oocytes expressing RME-2 could be observed when the control (empty vector (RNAi)) was compared to cdt-2(RNAi) animals (D and E). Arrows indicate oocytes.