Figure 4

CDT-2 can interact physically with SEM-5, the C. elegans GRB2 homologue. Pull down using in vitro labelled CDT-2 and purified GST, GST::SLI-1 or GST::SEM-5 shows that SEM-5 but not SLI-1 or GST can interact with CDT-2. As an additional negative control, we used labelled luciferase and did not detect interactions with either SEM-5 or SLI-1. Input is set at 5%.