Figure 4

Analysis of retested genes. A. Retested genes (from selected set, Figure 2C area a) that show significantly reduced signaling when down regulated by RNAi. Signals are shown as (+/-) percent deviation from the control RNAi signal. Three general classes are shown. All three classes down regulate both soluble (Nicd) and membrane bound (NΔecn) Notch-induced signal, yet have different effects on the E(spl)m3 promoter in the absence of active Notch. Class I genes have positive, Class II neutral and Class III negative effect on the uninduced signal. B. Selected set of retested genes that show significantly enhanced signaling when down regulated by RNAi. Two classes of hits are noted. Class IV is only effective on the membrane bound form of Notch (NΔecn), while class V is effective on both membrane bound and soluble forms (Nicd). All deviations are calculated to be significant by two-tailed t-test with p-values < 0.05 from control RNAi treatment (Additional file 5 for full statistics).