Figure 3

Protein-protein interaction map of Notch transcription modifiers. The Notch interaction network was generated by connecting the Notch transcription modifiers identified in the genome-wide study with protein-protein interaction links (e.g. two-hybrid and Co-IP data from the DroID database [19]). This resulting network included 126 genes (nodes) with 237 physical interactions (edges). Genetic interactions were not used for the network and the resulting map was drawn using Cytoscape [51]. A. These physical links are shown in relation to components of the activated Notch pathway (N and Su(H)) and the Notch repressor complex (Su(H), H, CtBP and gro), shown in red. B. Expanded view of the chromatin factors identified in this study that form the central core of the interaction network (blue). C. Ttk is a known downstream target of Notch signaling. The transcriptional and physical interaction data suggests that this factor may have a positive feedback role in Notch induced transcription. D. Factors with roles in mRNA processing (yellow). The interaction network suggests that these proteins may be working though the chromatin machinery to modulate Notch transcription. E. The interaction network suggests the possibility of a similar chromatin based mechanism for the class of ribosomal proteins known as Minute. The network file is included with the supplemental data (Additional file 6) and can be viewed in detail using the open source Cytoscape viewer http://www.cytoscape.org.