Endogenous expression of ASLV viral proteins in specific pathogen free chicken embryos: relevance for the developmental biology research field

Table 3 Detection of genomic ev loci regions in SPF embryos by PCR

Flock	Embryo	p27 region	Pol-env junction	Gp85 E env	IHC
1	1	+	+	+	na
	2	+	-	-	na
	3	+	+	-	na
	4	-	-	-	na
	5	+	+	-	na
2	1	+	+	+	+
	2	+	-	-	+
	3	+	+	-	na
	4	+	+	-	na
	5	+	+	+	na
	6	+	+	-	na
3	1	+	+	-	-
	2	+	+	+	-
	3	+	+	+	-
	4	+	+	+	-
	5	+	+	+	-
	6	+	+	+	na
	7	+	-	+	na
	8	+	+	-	na
4	1	+	+	-	-
	2	+	-	-	+
	3	+	+	+	-
	4	+	+	+	+
	5	+	+	+	-
	6	+	+	-	+
	7	+	+	+	-

Amniotic fluid samples were used to screen for the presence of three different regions of the ev loci by PCR. Presence of these sequences in the genome varied between flocks and between embryos among the same flock. Some embryos showed all three ev loci regions while others showed different combinations of two or only one of the regions. Twenty-five out of twenty-six embryos were positive for the p27 DNA region. No obvious correlation between composition of ev loci and detection of p27 expression by immunohistochemistry could be determined. IHC: immunohistochemistry. na: not available.

ISSN: 1471-213X

Flock	Embryo	p27 region	Pol-env junction	Gp85 E env	IHC
1	1	+	+	+	na
	2	+	-	-	na
	3	+	+	-	na
	4	-	-	-	na
	5	+	+	-	na
2	1	+	+	+	+
	2	+	-	-	+
	3	+	+	-	na
	4	+	+	-	na
	5	+	+	+	na
	6	+	+	-	na
3	1	+	+	-	-
	2	+	+	+	-
	3	+	+	+	-
	4	+	+	+	-
	5	+	+	+	-
	6	+	+	+	na
	7	+	-	+	na
	8	+	+	-	na
4	1	+	+	-	-
	2	+	-	-	+
	3	+	+	+	-
	4	+	+	+	+
	5	+	+	+	-
	6	+	+	-	+
	7	+	+	+	-

Flock	Embryo	p27 region	Pol-env junction	Gp85 E env	IHC
1	1	+	+	+	na
	2	+	-	-	na
	3	+	+	-	na
	4	-	-	-	na
	5	+	+	-	na
2	1	+	+	+	+
	2	+	-	-	+
	3	+	+	-	na
	4	+	+	-	na
	5	+	+	+	na
	6	+	+	-	na
3	1	+	+	-	-
	2	+	+	+	-
	3	+	+	+	-
	4	+	+	+	-
	5	+	+	+	-
	6	+	+	+	na
	7	+	-	+	na
	8	+	+	-	na
4	1	+	+	-	-
	2	+	-	-	+
	3	+	+	+	-
	4	+	+	+	+
	5	+	+	+	-
	6	+	+	-	+
	7	+	+	+	-

Flock	Embryo	p27 region	Pol-env junction	Gp85 E env	IHC
1	1	+	+	+	na
	2	+	-	-	na
	3	+	+	-	na
	4	-	-	-	na
	5	+	+	-	na
2	1	+	+	+	+
	2	+	-	-	+
	3	+	+	-	na
	4	+	+	-	na
	5	+	+	+	na
	6	+	+	-	na
3	1	+	+	-	-
	2	+	+	+	-
	3	+	+	+	-
	4	+	+	+	-
	5	+	+	+	-
	6	+	+	+	na
	7	+	-	+	na
	8	+	+	-	na
4	1	+	+	-	-
	2	+	-	-	+
	3	+	+	+	-
	4	+	+	+	+
	5	+	+	+	-
	6	+	+	-	+
	7	+	+	+	-