Figure 4From: Endogenous expression of ASLV viral proteins in specific pathogen free chicken embryos: relevance for the developmental biology research fieldRecommended workflow for developmental studies involving chicken embryos and RCAS vectors. SPF eggs should be selected by screening for lack of ASLV viral particles and endogenous viral protein expression in tissues. Afterwards, routine screening of selected lines is necessary. During experimental analysis, experimental infection should be determined by expression of viral proteins specific for the subgroup corresponding to the RCAS vector used, or alternatively, by using expression reporters such as epitope tags or fluorescent proteins.Back to article page