Figure 5

Visualization of insertions on genome browsers. (A) The zTrap genome browser showing a view surrounding the HG21C insertion. The HG21C insertion (a pink bar) is located within the first exon of the tcf7 gene [9] that are represented by multiple tracks below forward and reverse genomic sequences (two blue lines in the center), such as D. rerio cDNA, Ensembl transcripts and EST transcripts. Note that transcripts above the genomic sequences go from left to right and transcripts below them go in the opposite direction.

(B) The Ensembl genome browser showing the HGn8H insertion [9]. A track for zTrap insertions can be attached on the Ensembl genome browser as follows; (1) from the "region in detail" display page, select "configure this page" on the side bar, (2) select "custom data" on the top bar, (3) select "Attach DAS", (4) type "ztrap" in the "Filter sources" box and click "Next", (5) check the "zTrap" check box, click "Next", and close the pop-up window.