Figure 1

Pdlim7 is required for pectoral fin development. A-C: Whole-mount in situ hybridization using antisense RNA to show expression of pdlim7 in the developing pectoral fin mesenchyme at 33 hpf (A), 48 hpf (B), and 72 hpf (C). D-E: Sectioned embryos at 48 hpf of whole-mount in situ hybridization of pdlim7 show expression in fin mesenchyme. Boxed region in D is magnified in E to distinguish mesenchyme (purple color) and AER (arrow). F-G: Dorsal view of wild-type (F) and MO2 injected (G) embryos at 96 hpf. Arrows in G point to position of pectoral fin. H-J: Alcian blue stained cartilage preparations of dissected pectoral fins at 96 hpf. Wild-type (H), severe MO2 phenotype (I), and mild MO2 phenotype (J). I and J from same embryo. cl, cleithrum; pc, postcoracoid process; ed, endodermal disc; sco, scapulocoracoid.