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Figure 8 | BMC Developmental Biology

Figure 8

From: PLCγ-activated signalling is essential for TrkB mediated sensory neuron structural plasticity

Figure 8

TRPC3 is upregulated in TrkbPLC/PLC mutants. (A-B) Trpc isoform expression analysis in mouse adult vestibular tissue. Expression analysis of Trpc1-7 isoforms was performed by RT-PCR using as control total RNA preparations from a pool (A) consisting of E15 brain and retina and dissected vestibular ganglion tissue (B). All PCR products were verified by direct sequence analyses. The negative control was a PCR reaction with the total RNA template without the addition of reverse transcriptase. A 1 kb ladder was used as a standard size marker (STD) and the corresponding kb lengths were indicated. (C) RT-PCR for Trpc3 showed increased expression of this channel in the vestibular organ of TrkbPLC/PLC mutants compared to both controls (wt and TrkbWT/WT, p = 0.02). No difference was detected between the two controls (p = 0.5). (D) Neuron specific enolase (NSE) was used as an internal control specific to neurons. No significant difference was observed between the TrkbPLC/PLC mutants and the two controls.

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