Figure 5
From: PLCγ-activated signalling is essential for TrkB mediated sensory neuron structural plasticity
Afferent fibers are disorientated inside the vestibular sensory epithelia in absence of the TrkB/PLCγ docking site. (A-D) Shown are afferent fibers to the utricle labelled from the cerebellum; (A, C), P0 TrkbWT/WT control, and (B, D) P0 TrkbPLC/PLC point mutants. Note the highly focused projection in the control mice that already displays partial calyx formation (outlined by circles, A, C) with no fibers extending along the epithelial perimeters (dotted outline). In contrast, TrkbPLC/PLC mice show fibers extending for long distances along the epithelial perimeters (dotted outline) as well as within the sensory epithelium (arrows in panels B, D). There is only an occasional indication of partial calyx formation in TrkbPLC/PLC mutants at this stage (B, white circle). Scale bars, 100 μm in (A, B) and 10 μm in (C, D).