Skip to main content
Figure 2 | BMC Developmental Biology

Figure 2

From: Multispectral fingerprinting for improved in vivo cell dynamics analysis

Figure 2

A Comparison of Cell Identification in 1-Color and 3-Color Labeled Chick Embryo Tissues. (A-H) Four different groups of embryos HH St 9 (n = 5-6 in each set) were injected with 1-color (DiI, Gap43-GFP, or H2B-RFP) and 3-color (H2B-YFP, RFP, CFP or GFP) combinations. (A, C, E, G) After reincubation of 18-24 hours, the embryos were sectioned and typical confocal lambda and channel z-stacks were obtained. (B, D, F, H) The 3D reconstructions of each labeling method are shown with the corresponding segmented spot detection. (I) The graph highlights the differences between the cell identification for the different labeling strategies. The error bars show +/- one standard deviation. The scale bars are all 10 um.

Back to article page