Figure 2

Specificity of morpholino induced dmbx1a and dmbx1b knockdown using fusion protein constructs Dmbx1a-GFP and Dmbx1b-GFP. (A, B) Schematic diagrams showing sequence of fusion constructs: 5' UTR (black horizontal line); 5' end of the CDS for either dmbx1a or dmbx1b (white box), the full-length coding sequence for GFP (green box); site of polyadenylation (AAAA); relative position binding sites (red horizontal line) where the MOs block translation of the fusion proteins. (C-J) dmbx1a- and dmbx1b-GFP mRNA was either injected alone or co-injected with MOs as indicated at the bottom left of each panel, and embryos were examined for the presence of GFP fluorescence at 24 hpf (lateral view with anterior to the left). The percentage of GFP positive embryos is shown on the top right. UTR, untranslated region; CDS, coding sequence; MO, morpholino; mMO, mismatch morpholino; GFP, green fluorescent protein; hpf, hours post-fertilization.