Table 5 Sequences of primers and ISH probes.
From: Prediction of microRNAs affecting mRNA expression during retinal development
DNA oligonucleotides | |
|---|---|
Name | 5'-3' nucleotide sequence |
Let-7d | AGAGGTAGTAGGTTGCATAGT |
miR-9 | TCTTTGGTTATCTAGCTGTATGA |
miR-24 | TGGCTCAGTTCAGCAGGAACAG |
miR-25 | CATTGCACTTGTCTCGGTCTGA |
miR-27a | TTCACAGTGGCTAAGTTCCGC |
miR-34a | TGGCAGTGTCTTAGCTGGTTGTT |
miR-34b | TAGGCAGTGTAATTAGCTGATTG |
miR-122 | TGGAGTGTGACAATGGTGTTTG |
miR-124 | TAAGGCACGCGGTGAATGCC |
miR-128 | TCACAGTGAACCGGTCTCTTTT |
miR-125a-5p | TCCCTGAGACCCTTTAACCTGTGA |
miR-125b-5p | TCCCTGAGACCCTAACTTGTGA |
miR-130a | CAGTGCAATGTTAAAAGGGCAT |
miR-130b | CAGTGCAATGATGAAAGGGCAT |
miR-150 | TCTCCCAACCCTTGTACCAGTG |
miR-204 | TTCCCTTTGTCATCCTATGCCTG |
miR-326 | CCTCTGGGCCCTTCCTCCAGT |
miR-370 | GCCGGCTGGGGTGGAACGTGGTT |
miR-378 | CTCCTGACTCCAGGTCCTGTGT |
miR-485-5p | AGAGGCTGGCCGTGATGAATTC |
Poly (T) adapter | GCGAGCACAGAATTAATACGACTCACTATAGGTTTTTTTTTTTTWN |
Reverse RACE | GCGAGCACAGAATTAATACGAC |
ETS1F | CCAGACAGACACCTTGCAGA |
ETS1R | TGAGGCGATCACAACTATCG |
KLF13F | GAAGCACAAGTGCCACTACG |
KLF13R | GGCAGCTGAACTTCTTCTCG |
NFIBF | AGCTGCTGGAAGTCGAACAT |
NFIBR | TGAAGGTGGAGGTGGAGTTC |
LIN28BF | AAGGCCTTGAGTCAATACGG |
LIN28BR | CACTTCTTTGGCTGAGGAGG |
SH2B3F | CTGGAGCTCTTCGACCCAC |
SH2B3R | ATGTCTGTCCGGTCCTTCAC |
ACCN2F | AGCTGTTACCATGGACTCGG |
ACCN2R | CACGCAGTACTCCTGGTCCT |
in situ probes | |
Name | Sequence (enhanced with LNA) 5'-3' |
mmu-miR-124 | GGCATTCACCGCGTGCCTTA |
mmu-miR-34a | AACAACCAGCTAAGACACTGCCA |
mmu-miR-128 | AAAAGAGACCGGTTCACTGTGA |
mmu-miR-125b-5p | TCACAAGTTAGGGTCTCAGGGA |
mmu-miR-378 | CCTTCTGACTCCAAGTCCAGT |
mmu-miR-122 | CAAACACCATTGTCACACTCCA |
Scrambled-miR | TTCACAATGCGTTATCGGATGT |