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Figure 4 | BMC Developmental Biology

Figure 4

From: Developmental expression of survivin during embryonic submandibular salivary gland development

Figure 4

Spatial distribution of survivin protein during embryonic SMG development. A. Survivin protein is diffusely distributed throughout the branching epithelia (e) and absent from the mesenchyme (m) in the Pseudoglandular Stage. B. Intense survivin immunostain is seen in ductal columnar epithelial cells which surround forming lumina (arrow heads) in the Canalicular Stage. By contrast, survivin protein retains its diffuse distribution in terminal bud (t) epithelia lacking lumina and in outer ductal cuboidal epithelial cells. C-E. Terminal Bud Stage SMGs exhibit distinct lumina in presumptive ducts (d) and acini (a). Note intense immunostain in the inner layer of ductal epithelia and the single layer of terminal bud epithelia which surround distinct lumina . D. Higher magnification of Terminal Bud Stage SMG exhibiting nuclear localization (arrows) of survivin protein in presumptive acini cells bounding distinct lumina (*). E. Higher magnification of a Late Terminal Bud Stage ductal epithelial bilayer which consists of an inner columnar epithelia which surround the distinct lumen and an outer cuboidal epithelia which abuts the basement membrane. Nuclear localization (double arrowheads) of survivin protein is seen in the inner layer of columnar epithelial cells surrounding the lumen (*) and not in the outer cuboidal epithelia. A-C. Bar, 50 μm. D-E, Bar, 25 μm.

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