mec-3 upstream sequences that contribute to ALM migration defects. The diagrams on the left indicate the regions of mec-3 in each plasmid. The top diagram labeled mec-3 includes sequences from 2400 bp 5' of the mec-3 translational start site (ATG) through exon 3. PstI, HincII, BglII and HpaI indicate restriction enzyme cutting sites. Three lines were generated for each plasmid. The bar graphs show the positions of ALMs from 100 animals for each of the three lines. The percent of misplaced ALMs and the number of ALMs scored are shown on the right.