Figure 6

TNF-induced NF-κB nuclear translocation is inhibited by SN50 treatment. We evaluated the pattern of NF-κB p65 distribution in E15 SMG primordia cultured for 7 days in 10 U/ml rTNF (A) or 100 μg/ml SN50 + 10 U/ml rTNF (B) supplementation. In TNF-supplemented explants (A), NF-κB is detected in SMG terminal bud cell nuclei (arrow heads). By contrast, NF-κB is absent from cell nuclei (arrows) in TNF + SN50-supplemented explants (B). Rather, a weak, diffuse pattern of NF-κB immunolocalization in the cytoplasm is seen. Similar results were observed for NF-κB p50 immunolocalization (not shown). Bar, 100 μm.