Figure 3

Cell proliferation and apoptosis. E15 SMG primordia were cultured in the presence or absence of 100 μg/ml SN50 peptide for 2 days (E15 + 2) and cell proliferation and apoptosis was determined. A., B. Cell proliferation. There is a marked decrease in cell proliferation (PCNA positive/brown color) with SN50 treatment (B) compared to control (A). Note that these sections were counterstained in hematoxylin; thus the cytoplasm in non PCNA-positive cells appears blue. C., D. Apoptosis. SN50 treatment (D) induced a notable increase in apoptotic positive nuclei (dark brown color) in ductal and terminal bud epithelia compared to control (C). Note that since these sections were not counterstained; thus the cytoplasm appears light brown. Bar: 50 μm.