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Table 3 Development of porcine embryos reconstructed by transferring confluent (CON) or nocodazole-treated (NOC) cells into enucleated M I or M II oocytes

From: Development of porcine embryos reconstituted with somatic cells and enucleated metaphase I and II oocytes matured in a protein-free medium

   

No. of

No. of

No. (%)b of embryos developed to

Mean no. ± SEM

     

Mean no. ± SEM

  
  

No. of

complexes

complexes

≥ 2-cell

Blastocyst

of cells in

Recipient

Donor

trials

treated

fused (%)a

(2)c

(7)c

blastocysts

M I

CON

4

180

132 (73.3)d

7 (5.3)d

2(1.5)d

19.0 ± 5.0

 

NOC

4

179

72 (40.2)de

7 (9.7)d

0(0)d

-

M II

CON

3

227

131 (57.7)d

50 (38.2)e

10 (7.6)e

45.1 ± 6.2

 

NOC

3

136

39 (28.7)e

6 (15.4)de

0(0)d

-

  1. aPercentage of complexes treated. bPercentage of embryos cultured. cNumbers in parentheses indicate the time of examination (days after culture). d-eValues with different superscripts within each column are significantly different (P < 0.05).