Figure 4
From: Association of mesenchymal cells and immunoglobulins with differentiating epithelial cells
Dual color IHC (HLA-DR peroxidase/CD8 FITC) viewed in dark field visible light (A), incident fluorescence (B) and dark field fluorescence (C). A) Interface (dashed line) between pb and im layers. White arrowhead shows differentiating DC, yellow arrowhead shows mature DC. Arrow indicates activated T cell with HLA-DR expression (see below). B) White arrow indicates T cell exhibiting unusual elongated shape at the interface. Yellow arrows indicate residual CD8 expression in fragmented T cell among adjacent im epithelial cells. C) Activated T cell with HLA-DR expression (white arrow) interacts with differentiating DC (white arrowhead). Mature DC (yellow arrowhead) accompany T cell fragmentation (yellow arrows).