Gad1 expression in the developing tail. (A-F) Gad1 expression in the tail from E9.0-E12.5. (A) Gad1 is strongly expressed in the tail at E9.0. At E10.5 (B) and E11.5 (C), the expression becomes localized towards the tip of the tail. (D) By E12.5 Gad1 expression fades. (E, F) Sections through the tail of an E9.5 embryo hybridized to the Gad1 probe prior to sectioning. The plane of section in (E) is cranial to that shown in (F). The hindgut (hg), neural tube (nt), tailbud mesenchyme (m) and neuroepithelium (n) are indicated. Scale bar: A-D 750 μm; E 75 μm, F 100 μm.