Spatiotemporal association of DNAJB13 with the annulus during mouse sperm flagellum development
© Guan et al; licensee BioMed Central Ltd. 2009
Received: 25 October 2008
Accepted: 19 March 2009
Published: 19 March 2009
The sperm annulus is a septin-based fibrous ring structure connecting the midpiece and the principal piece of the mammalian sperm flagellum. Although ultrastructural abnormalities and functional importance of the annulus have been addressed in Sept4-null mutant mice and a subset of human patients with asthenospermia syndrome, little is known about how the structure is assembled and positioned to the midpiece-principal piece junction during mammalian sperm flagellum development.
By performing immunofluorescence and biochemical approaches with antibodies against DNAJB13 and an annulus constituent SEPT4, we report here a spatiotemporal association of DNAJB13 with sperm annulus during mouse sperm flagellum development. DNAJB13 co-localized with SEPT4 to the annulus, and both were first able to be detected in step 9 spermatids. As spermiogenesis proceeded, the annular DNAJB13 immunosignal increased until the annulus reached the midpiece-principal piece junction, and then gradually disappeared from it in late spermiogenesis. In contrast, the SEPT4 immunosignal was relatively unaltered, and still present on annulus of mature spermatozoa. In Sept4-null mouse spermatids lacking the annulus structure, the annulus-like DNAJB13 immunosignal was still able to be detected, albeit weaker, at the neck region of the flagella. In vitro DNAJB13 was co-localized and interacted with SEPT4 directly.
The direct interaction of DNAJB13 with SEPT4 in vitro and its spatiotemporal association with the annulus during sperm flagellum development, and even its annulus-like appearance in the annulus-deficient spermatids, suggest that DNAJB13 may be involved in assembling the annulus structure and positioning it towards the midpiece-principal piece junction.
The sperm annulus is a ring-like structure existing in all mammalian spermatozoa. This structure was identified 100 years ago and formerly called "Jensen's ring". At low magnification, the annulus appears dense and homogeneous, but at high magnification it shows to be composed of closely packed filamentous subunits oriented circumferentially .
During early spermiogenesis, a flagellar axoneme forms beneath the cell surface from one of the two centrioles, and rapidly protudes from the cell. The centriole pair forming the axoneme then move towards the nucleus and finally impacts it, which causes the cell membrane adherent to the centriole to become infolded . As early as in this stage the annulus or the anlage of the annulus can be seen encircling the axoneme at the distal end of the basal body by electron microscopy [3, 4]. During the late stage of sperm flagellum development, the annulus slips towards a more distal position, and the mitochondria begin to affix to the flagellum. In a mature spermatozoon the annulus is located between the midpiece and the principal piece of the flagellum, and beneath the plasma membrane, firmly connecting them together [1, 3, 5–7].
Recent researches show that the sperm annulus is a septin-based structure composed of several septins, such as SEPT1, 4, 6, 7 and 12 [8–10]. Septins, conserved from yeast to Drosophila to humans, are polymerizing GTPases that can form hetero-oligomeric filaments required for cytokinesis and other cellular processes [11, 12]. The functional importance of septins in sperm annulus formation is illustrated by the Sept4-deficient male mice. Sept4-deficient male mice are sterile due to defective morphology and motility of the sperm flagella. Mutant sperm lacking the annulus often show bent-back tail morphology at the midpiece-principal piece junction. Therefore, cortical organization of the annulus based on circular assembly of septins is essential for the structural and mechanical integrity of mammalian spermatozoa [8, 9]. To date, however, little is known about how the annulus/septin ring is assembled and positioned to the midpiece-principal piece junction during spermiogenesis. In vivo, mammalian septins may need extra factors or adaptors to organize higher-order structures, albeit their self-assembly into filamentous rings in vitro . Thus, the present study intends to examine the involvement of DNAJB13, a type II heat shock protein 40 (HSP40), in annulus assembly and positioning during mammalian sperm flagellum development.
HSP40s generally function as cochaperones in the HSP70 chaperone systems that are involved in many processes in cells, such as folding of newly synthesized proteins, refolding of misfolded proteins, translocation of proteins into organelles, assembly and disassembly of protein complexes, and protein degradation [14–16]. We have previously shown that DNAJB13 is a component of mouse sperm axoneme . Here we report that DNAJB13 is associated with the annulus spatiotemporally during sperm flagellum development in wild type mice, and even present temporarily in the annulus-deficient Sept4-null spermatids taking on an annulus-like pattern. In vitro DNAJB13 interacts with SEPT4 directly. Altogether, our data suggest that DNAJB13 may assist in assembling the annulus and positioning it toward the midpiece-principal piece junction during sperm flagellum development.
Results and discussion
DNAJB13 localized to sperm annulus transiently during mouse spermiogenesis
Due to the difference in signal intensity and angles of view, the annular DNAJB13 staining exhibited three distinct patterns. In most cases, it showed a bar-like pattern (Figure 1A; figure 3; additional file 2B); but the ring-like pattern (Figure 1D; additional file 1; additional file 2C) was obvious when detached from flagella; it also displayed a two-dot pattern when the signal intensity was low, especially at the beginning of the annulus formation (Additional file 2A). In addition to its annulus location, DNAJB13 staining on the same testicular cell preparation uncovered a discernible pattern. In a subset of spermatids, a small dot labelled with the antibody was kept apart from the annulus staining toward the distal end of the sperm flagellum (Figures 1A, C). During sample preparation, some spermatids were damaged and their annuli were released. In this case, DNAJB13 was still co-localized with SEPT4 on the ring-like structure (Figure 1D), indicating that there may be a strong physical interaction between them.
Spatiotemporal progression of annular DNAJB13 during spermiogenesis
Intrigued by our observation above, we employed immunofluorescence microscopy to follow the spatiotemporal progression of annular DNAJB13 with the annulus during mouse spermiogenesis in detail. In order to define each step in spermiogenesis, we combined the antibody staining with DAPI (nuclear staining) and MitoTracker (mitochondrial marker). Because it is difficult for us to demonstrate 4 colors in one sample, we stained the testicular germ cells with the SEPT4 antibody and the DNAJB13 antibody separately.
Localization of DNAJB13 in Sept4-null spermatids
As in wild type, the DNAJB13-positive dot was able to be detected on flagella of the Sept4-null spermatids, and also migrated towards the midpiece-principal piece junction (Figure 4A). Therefore, its presence and migration are likely to be independent of annulus. Since the mitochondrial sheath is still formed in Sept4-null spermatids [8, 9], the dot may play a role in mitochondrial sheath orientation in the absence of annulus.
In vitro DNAJB13 co-localized and interacted with SEPT4
In an effort to determine whether DNAJB13 and SEPT4 interact in vivo, we first made the testicular lysate. However, the great majority of testicular SEPT4 was detergent-insoluble (data not shown). We then turned to an in vitro assay by constructing plasmids expressing GFP-tagged DNAJB13 and FLAG-tagged SEPT4 respectively, co-transfecting them into HeLa S3 cells, and then performing both immunofluorescence and co-immunoprecipitation (Co-IP) analyses.
By performing immunofluorescence and biochemical approaches, we for the first time revealed a spatiotemporal association of DNAJB13, a type II HSP40, with the sperm annulus and elucidated a relationship between DNAJB13 and the annular constituent SEPT4. With closer inspection of the annulus-deficient spermatids from Sept4-null mice, we here tentatively propose a model of how DNAJB13 is involved in the assembly and positioning of sperm annulus. In the early stage of sperm flagellum development, DNAJB13 forms an annular anlage, or attaches to a pre-existing annular anlage, and then recruits septins through direct interaction with SEPT4 or SEPT4-containing septin complex to build up a genuine annulus. In the late stage of sperm flagellum development, DNAJB13 may assist the migration of the annulus, and meanwhile forms a dot on the flagellum to direct the annulus toward the midpiece-principal piece junction. As the annulus is positioned to the midpiece-principal piece junction, DNAJB13 disappears gradually with formation and maturation of the mitochondrial sheath.
All animal procedures were approved by Stockholm-North Animal Ethical Committee or the Animal Care and Use Committee of Kyoto University. C57BL/6N mice were purchased from Charles River, Germany. Sept4+/- mice were backcrossed with C57BL/6J for more than 16 generations. Sept4-/- mice and the control littermates were generated from Sept4+/- mice.
5' ATCTCGAGCCATGGGGCTGGATTACT 3'
5' ATAAGCTTGGTCAGCAATGCCTGGCG 3'
5' AAGTCGACCATTCACTGGGATGG 3'
5' ATGGATCCTTAGTGAGTCTCCTTCATCTG 3'
In vitro studies
HeLa S3 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) in an atmosphere containing 5% CO2 at 37°C. Cells were transfected with Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer's instruction.
For immunofluorescence analysis p3xFLAG-Sept4 was co-transfected with pEGFP-Dnajb13 into HeLa S3 cells plated onto coverslips. Twenty-four hours later these coverslips were fixed and subjected to immunofluorescence analysis.
For Co-IP analysis, plasmids p3xFLAG-Sept4 and pEGFP-Dnajb13 were co-transfected into HeLa S3 cells plated in a 10-cm dish. Twenty-four hours later cells were washed once with 1× PBS and then lysed with ELB lysis buffer (50 mM Hepes, pH 7.4, 0.25 M NaCl, 0.5 mM EDTA, 0.1% NP_40) supplemented with 1× protease inhibitor cocktail (Roche) on ice for 30–60 minutes. After lysis the cells were scraped off and transferred into a new eppendorf tube. After centrifuge at 13,000 rpm for 20 minutes at 4°C, a small aliquot of the supernatant was taken as input, and the left was divided into two new eppendorf tubes equally. One tube was added with rabbit anti-GFP antibody (A11122, Invitrogen), and the other was added with normal rabbit IgG. After 2-hour incubation at 4°C with gentle rotation, each tube was added with protein G beads (Upstate) and incubated for another 2 hours. After 4 times wash with lysis buffer, the beads were boiled in 2 × SDS sampling buffer at 95°C for 10 minutes and then centrifuged at 13,000 rpm for 2 minutes to collect the Co-IP products. The input and the Co-IP products were then subjected to immunoblotting analysis.
For germ cells, adult mouse testes or epididymes were collected and minced in 1 × PBS to release germ cells. After filtration the cells were attached onto acid-treated, poly-L-lysine-coated coverslips. The coverslips were then fixed in 4% paraformaldehyde (PFA) for 15 minutes and permeabilized in 0.25% Triton X-100 for 10 minutes at room temperature. After blocking in 3% BSA the coverslips were incubated with the primary antibodies for 1–2 hours at room temperature. After rinse the coverslips were then incubated with the secondary antibodies alone or together with MitoTracker Red 580FM (Molecular Probes, Invitrogen) for 30 minutes at room temperature. After rinse and DAPI staining, the coverslips were mounted onto slides with Prolong anti-fade reagents (Molecular Probes, Invitrogen). The primary antibodies used were rabbit anti-DNAJB13 antibody (1:50), rabbit anti-SEPT4 antibody (Santa Cruz, 1:200) and guinea pig anti-SEPT4 antibody (raised against oligopeptide corresponding to residues 466–478 of mouse SEPT4, 1:100). The secondary antibodies used were FITC-conjugated swine anti-rabbit IgG (Dako, 1:100) and Cy3-conjugated donkey anti-guinea pig IgG (Jackson Lab, 1:1,200).
For cultured cells, the coverslips were fixed and permeabilized in 2% PFA + 0.5% Triton X-100 in 1× PBS for 15 minutes at room temperature. The following steps were performed as described above. The primary antibody used was rabbit anti-SEPT4 antibody (Santa Cruz, 1:200). The second antibody was TRITC-conjugated goat anti-rabbit IgG (Santa Cruz, 1:100).
All the slides were examined using a Leica DMRA2 microscope (Leica Corp.), and photographed with Hamamatsu ORCA-HR digital camera (Hamamatsu, Japan) operated by OpenLab software (ImproVision Inc.)
Immunoblotting was performed as described previously . Briefly, protein samples including input and Co-IP products were separated by SDS-polyacrylamide gel electrophoresis (PAGE) and transferred to PVDF membrane. The membrane was blocked with 5% non-fat milk in 1× TBS (blocking buffer) for 1 hour at room temperature and then incubated with primary antibodies in blocking buffer overnight at 4°C. After rinse the membrane was incubated with secondary antibodies in blocking buffer for 1 hour at room temperature. The immunosignal was detected with the LAS-1000 plus image analyzer (FUJIFILM) using ECL kit (Pierce, Rockford, IL). The primary antibodies used were mouse anti-FLAG M2 monoclonal antibody (F-3165, Sigma, 1:20,000), and mouse anti-GFP monoclonal antibody (A11120, Invitrogen, 1:2,000). The secondary antibody was HRP-conjugated goat anti-mouse IgG antibody (DAKO, 1:5,000).
We thank Prof. Christer Höög for generously providing us with the Leica DMRA2 microscope with Openlab™ software. This work has been supported by the Swedish Research Council, the ÅKE WIBERGS Foundation and Karolinska Institutet.
- Fawcett DW: A comparative view of sperm ultrastructure. Biol Reprod. 1970, 2: 90-127.View ArticlePubMedGoogle Scholar
- Russell LD, Ettlin RA, SinhaHikim AP, Clegg ED: Histological and histopathological evaluation of the testis. 1990, Cache River Press, Florida, 20-22, 90, 98, 132, 143, 152.Google Scholar
- Yasuzumi G, Shiraiwa S, Yamamoto H: Spermatogenesis in animals as revealed by electron microscopy. XXVII. Development of the neck region and flagellum of the cat spermatozoon. Z Zellforsch Mikrosk Anat. 1972, 125: 497-505.View ArticlePubMedGoogle Scholar
- Fawcett DW, Eddy EM, Phillips DM: Observations on the fine structure and relationships of the chromatoid body in mammalian spermatogenesis. Biol Reprod. 1970, 2: 129-53.View ArticlePubMedGoogle Scholar
- Burgos MH, Fawcett DW: Studies on the fine structure of mammalian testis. I. Differentiation of the spermatids in the cat (Felis domestica). J Biophys Biochem Cytol. 1955, 1: 287-300.PubMed CentralView ArticlePubMedGoogle Scholar
- Fawcett DW: The structure of the mammalian spermatozoon. Int Rev Cytol. 1958, 7: 195-234.View ArticleGoogle Scholar
- Fawcett DW: The mammalian spermatozoon. Dev Biol. 1975, 44: 394-436.View ArticlePubMedGoogle Scholar
- Ihara M, Kinoshita A, Yamada S, Tanaka H, Tanigaki A, Kitano A, Goto M, Okubo K, Nishiyama H, Ogawa O, et al: Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa. Dev Cell. 2005, 8: 343-52.View ArticlePubMedGoogle Scholar
- Kissel H, Georgescu MM, Larisch S, Manova K, Hunnicutt GR, Steller H: The Sept4 septin locus is required for sperm terminal differentiation in mice. Dev Cell. 2005, 8: 353-64.View ArticlePubMedGoogle Scholar
- Steels JD, Estey MP, Froese CD, Reynaud D, Pace-Asciak C, Trimble WS: Sept12 is a component of the mammalian sperm tail annulus. Cell Motil Cytoskeleton. 2007, 64: 794-807.View ArticlePubMedGoogle Scholar
- Kinoshita M: Diversity of septin scaffolds. Curr Opin Cell Biol. 2006, 18: 54-60.View ArticlePubMedGoogle Scholar
- Lindsey R, Momany M: Septin localization across kingdoms: three themes with variations. Curr Opin Microbiol. 2006, 9: 559-65.View ArticlePubMedGoogle Scholar
- Kinoshita M, Field CM, Coughlin ML, Straight AF, Mitchison TJ: Self- and actin-templated assembly of Mammalian septins. Dev Cell. 2002, 3: 791-802.View ArticlePubMedGoogle Scholar
- Qiu XB, Shao YM, Miao S, Wang L: The diversity of the DnaJ/Hsp40 family, the crucial partners for Hsp70 chaperones. Cell Mol Life Sci. 2006, 63: 2560-70.View ArticlePubMedGoogle Scholar
- Mayer MP, Bukau B: Hsp70 chaperones: cellular functions and molecular mechanism. Cell Mol Life Sci. 2005, 62: 670-84.PubMed CentralView ArticlePubMedGoogle Scholar
- Bukau B, Horwich AL: The Hsp70 and Hsp60 chaperone machines. Cell. 1998, 92: 351-66.View ArticlePubMedGoogle Scholar
- Guan J, Yuan L: A heat-shock protein 40, DNAJB13, is an axoneme-associated component in mouse spermatozoa. Mol Reprod Dev. 2008, 75: 1379-86.View ArticlePubMedGoogle Scholar
- Kato A, Nagata Y, Todokoro K: Delta-tubulin is a component of intercellular bridges and both the early and mature perinuclear rings during spermatogenesis. Dev Biol. 2004, 269: 196-205.View ArticlePubMedGoogle Scholar
- Campbell PK, Waymire KG, Heier RL, Sharer C, Day DE, Reimann H, Jaje JM, Friedrich GA, Burmeister M, Bartness TJ, et al: Mutation of a novel gene results in abnormal development of spermatid flagella, loss of intermale aggression and reduced body fat in mice. Genetics. 2002, 162: 307-20.PubMed CentralPubMedGoogle Scholar
- Sha B, Lee S, Cyr DM: The crystal structure of the peptide-binding fragment from the yeast HSP40 protein Sis1. Structure. 2000, 8: 799-807.View ArticlePubMedGoogle Scholar
- Stirling PC, Bakhoum SF, Feigl AB, Leroux MR: Convergent evolution of clamp-like binding sites in diverse chaperones. Nat Struct Mol Biol. 2006, 13: 865-870.View ArticlePubMedGoogle Scholar
- Yang C, Compton MM, Yang P: Dimeric novel HSP40 is incorporated into the radial spoke complex during the assembly process in flagella. Mol Biol Cell. 2005, 16: 637-648.PubMed CentralView ArticlePubMedGoogle Scholar
- Guan J, Ekwurtzel E, Kvist U, Yuan L: Cohesin protein SMC1 is a centrosomal protein. Biochem Biophys Res Commun. 2008, 372: 761-764.View ArticlePubMedGoogle Scholar
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