Protein Kinase D regulates several aspects of development in Drosophila melanogaster
© Maier et al; licensee BioMed Central Ltd. 2007
Received: 24 November 2006
Accepted: 25 June 2007
Published: 25 June 2007
Protein Kinase D (PKD) is an effector of diacylglycerol-regulated signaling pathways. Three isoforms are known in mammals that have been linked to diverse cellular functions including regulation of cell proliferation, differentiation, motility and secretory transport from the trans-Golgi network to the plasma membrane. In Drosophila, there is a single PKD orthologue, whose broad expression implicates a more general role in development.
We have employed tissue specific overexpression of various PKD variants as well as tissue specific RNAi, in order to investigate the function of the PKD gene in Drosophila. Apart from a wild type (WT), a kinase dead (kd) and constitutively active (SE) Drosophila PKD variant, we also analyzed two human isoforms hPKD2 and hPKD3 for their capacity to substitute PKD activity in the fly. Overexpression of either WT or kd-PKD variants affected primarily wing vein development. However, overexpression of SE-PKD and PKD RNAi was deleterious. We observed tissue loss, wing defects and degeneration of the retina. The latter phenotype conforms to a role of PKD in the regulation of cytoskeletal dynamics. Strongest phenotypes were larval to pupal lethality. RNAi induced phenotypes could be rescued by a concurrent overexpression of Drosophila wild type PKD or either human isoform hPKD2 and hPKD3.
Our data confirm the hypothesis that Drosophila PKD is a multifunctional kinase involved in diverse processes such as regulation of the cytoskeleton, cell proliferation and death as well as differentiation of various fly tissues.
Protein kinases D (PKD) are serine/threonine-specific kinases that belong to the subfamily of Ca(2+)/Calmodulin kinases. They are effectors in diacylglycerol-regulated signaling pathways. In mammals, three highly related PKDs 1–3 (in human named also PKCμ, PKD2 and PKCν) are known . PKD contains two domains, a regulatory domain and a catalytic kinase domain. The regulatory domain inhibits the kinase domain, until the enzyme is activated by phosphorylation of two serine residues located within the kinase domain. Three ways have been described to activate PKD1 . As consequence of a mitotic signal, diacylglycerol is generated by phospholipase C stimulation, resulting in the activation of either novel protein kinase C, PKCε or PKCη, which in turn phosphorylate PKD1 [3, 4]. Alternatively, PKD1 is activated by Gβγ that binds to the regulatory domain, thereby abrogating its inhibitory function . Finally, in response to genotoxic stress, the kinase domain can be released by Caspase-mediated cleavage [6, 7]. However, a PKD homologue in C. elegans DKF-1 (D-kinase factor 1) is directly activated by phorbol-esters independent of PKC . PKDs are found within different subcellular compartments in agreement with their multiple biological roles in highly diverse cellular processes including cell proliferation and apoptosis, cell migration, cellular differentiation and notably, cargo specific secretory transport from the trans-Golgi network (TGN) to the plasma membrane [1, 2]. The involvement of PKD in the regulation of fission of secretory vesicles from TGN was deduced primarily from overexpression experiments of a presumptive dominant negative PKD variant, which bears a single amino acid substitution in the ATP binding domain and therefore lacks kinase activity ('kinase dead') [9–11]. 'Kinase dead' PKD interferes with the fission of vesicles at the TGN owing to a tubularization of Golgi membranes . The relevance of these observations is strengthened by the finding that one of the physiological substrates of PKD1 and PKD2 is phosphatidylinositol-4 kinase III beta (PI4KIIIβ), which is central to Golgi structure and function . Apart from its role in secretory transport, transgenic mouse models reveal the importance of PKD for differentiation of T lymphocytes . Moreover, mutants of the corresponding C. elegans kinase DKF-1 displayed body paralysis, whereas overexpression caused growth defects .
The Drosophila genome harbors a single PKD homologue. As expected for a multifunctional protein, PKD is broadly expressed during development. A fraction of the PKD protein localizes to the Golgi compartment in agreement with a proposed role in secretory transport . Hence, Drosophila may serve as model system to investigate the in vivo function of PKD. To this end, we have analyzed the phenotypic consequences of overexpression of wild type and mutant PKD variants on the development of a number of tissues, the consequences of tissue specific RNA-interference and the capacity of human hPKD2 and hPKD3 to substitute for Drosophila PKD. Only slight defects primarily during wing vein development were observed upon overexpression of wild type (WT) or kd-variants, whereas overexpression of a constitutively active form is deleterious to fly development, as is PKD RNAi. Our data are in accordance with a role for PKD in the regulation of cytoskeletal dynamics, cell proliferation and death and hence, the differentiation of various tissues during fly development.
PKD overexpression constructs
Activity of PKD-kd and PKD-SE constructs in human cell culture
Overexpression of PKD interferes with wing development
Ubiquitous overexpression of PKD-SE and dsPKD disrupted fly development. Offspring from a cross of da-Gal4 with UAS-PKD-SE all died at first larval instar larva at 18°C and at 25°C. Most da > dsPKD animals died as prepupae at 25°C and only few started pupation (13 of 207); at 18°C they developed into pharate adults with few attempting to hatch (5 of 164). Induced within the posterior compartment only, PKD-SE was fully lethal at late pupal stage at 25°C and semilethal at 18°C (32 of 224 animals hatched). Many of the eclosed animals were defective in wing inflation; unfolded wings typically showed a complete lack of cross veins (Fig. 3F). Hence, cross vein formation requires a tight regulation of PKD activity. The ectopic veins that were caused by overexpression of PKD-kd may reflect dominant negative effects.
PKD specific RNA interference during larval development results in loss of tissue
Human PKD can rescue loss of DrosophilaPKD activity
Human PKD can replace Drosophila PKD activity
Manipulation of PKD activity results in degeneration of the adult retina
Rescue of external eye phenotypes by Drosophila inhibitor of apoptosis DIAP1
2) DIAP1 + PKD-SE
4) DIAP1 + dsPKD
Age dependency of external eye phenotypes
gmr > PKD-SEa
gmr > dsPKDb
≥ 3 spots
Sections of the adult eyes revealed retina defects for both genotypes (see below). We noted thinner lenses in some ommatidia of gmr > dsPKD flies (Fig. 6B). The large black patches in older flies were filled with cocci (Fig. 6D). Bacterial infection can be an explanation for the darkening and increase in size of these patches with age and their resistance to rescue by DIAP1 (Fig. 6D,E; Tables 2, 3). The observed lesions in the eye lens may damage the physical barrier of the cuticle, thereby allowing bacterial entry more easily. Accordingly, other obvious infections were not prevalent in dsPKD flies compared to their untreated siblings. Alternatively, PKD could be involved in fly immune response. However, no rescue was seen with UAS-defensin, which has been shown to specifically combat gram+bacteria .
Retina defects were analyzed in sections. PKD-SE overexpression induced striking elongation and fragmentation of rhabdomeres (Fig. 6B), whereas PKD-RNAi resulted in a collapse and disappearance of rhabdomeres (Fig. 6D). Conspicuous holes were detected between the ommatidia in both cases (Fig. 6B,D). Since the overall ommatidial architecture appeared quite normal at low resolution, we conclude that the retina degenerated during metamorphosis after being established normally during larval and early pupal development. Accordingly, photoreceptor cells appeared normal when visualized with anti-Elav antisera in third instar larval eye discs of gmr > PKD-SE animals (data not shown). Sections revealed a slight improvement by concomitant overexpression of DIAP1; most notably the holes disappeared (Fig. 6C,E). However, DIAP1 had little effect on the morphology of the rhabdomeres. Neither could it halt the fragmentation caused by overexpression of PKD-SE nor the disintegrating resulting from PKD-RNAi (Fig. 6C,E). Altogether, these data suggest that the degeneration of the adult retina is partly caused by apoptosis, yet other mechanisms must contribute as well. This was unexpected since retinal degeneration caused for example by rhodopsin mutations can be prevented by blocking apoptosis .
DrosophilaPKD affects wing development
Overexpression of both wild type and the kd-PKD isoforms interfered in particular with wing vein development. Wing veins are established in several phases during larval and pupal development and require the activity of a number of signaling pathways [28–30]. One example is the Notch signaling pathway. Overactivation of this pathway results in loss of veins indistinguishable of phenotypes observed for example in en > PKD-kd (Fig. 3E) [31, 32]. However, ectopic expression of proneural proteins can induce ectopic veinlets similar to what is seen in the da > PKD-kd wings . Notch activity reduces those veinlets and hence, acts in the opposite direction. Therefore, PKD might positively regulate Notch signaling, whereas the dominant negative activity of PKD-kd might inhibit it. Similar phenotypes can also be a consequence of disturbance of EGFR-signaling. For example, loss of veins is seen in veinlet (ve) mutants whereas overexpression of ve induces ectopic veins [31, 33]. The Ve protein is a positive regulator of EGFR-signaling and is required for the release of the respective ligands . Hence, PKD might be involved in the negative regulation of EGFR-signaling. Finally, the dpp-pathway plays an important role in the positioning and consolidation of veins and notably, of cross veins [28–30, 35–37]. Interestingly, vein phenotypes caused by an overactivation of the dpp-pathway are indistinguishable from those caused by the overexpression of PKD-kd . Any of these pathways or all of them may be influenced by PKD, which can be now addressed in more detail. As cross veins are most sensitive towards overexpression of PKD, the dpp-pathway seems to be the most promising candidate. The altered wing texture caused by overexpression of dsPKD appears to be non-autonomous and may reflect defects in the secretion of extracellular matrix or wing cuticle. Shortly after eclosion, wing epithelial cells delaminate and migrate into the thorax. Extra-cellular matrix components are produced presumably by these migrating cells that bond ventral and dorsal wing surfaces once the cells have disappeared . Disturbance of this process interferes with wing unfolding and bonding . The resultant phenotypes are reminiscent of defects seen upon overexpression of either PKD-SE (wing unfolding) or dsPKD (flaccid wings) and in agreement with a role for PKD in these processes.
Human PKD can replace DrosophilaPKD activity
Mammals contain three highly related PKD isoforms that, based on their similarity, may be functionally redundant. However, they are differentially expressed suggesting tissue specific activities . The human isoforms, hPKD2 and hPKD3 rescued the wing phenotype caused by RNAi with Drosophila PKD (Table 1). Drosophila and human PKDs show an overall degree of similarity of about 65%, albeit the kinase domain is well-conserved . The latter raises the possibility of an interference between dsPKD and hPKD mRNA. Stretches of identical sequences are rather short, however, may suffice for interference notably with hPKD2 and less likely with hPKD3 (see Materials and Methods) . The similarity in rescue of either construct is in agreement with a replacement of Drosophila PKD function rather than a titration of the generated siRNAs by hPKD mRNA. Strikingly, we observed that both hPKD proteins were predominantly cytosolic in Drosophila tissues, just like the Drosophila PKD protein (Fig. 1B–E). In contrast to Drosophila PKD, hPKDs are nuclear and cytosolic in mammalian cells [2, 13]. Mammalian cells use specific mechanisms to regulate nuclear import of PKD that may not operate in the fly . Nuclear transport of PKD requires PKC and responds to G protein coupled receptor (GPCR) activation . In this context, it is noteworthy that the C. elegans homologue DKF-1 is stimulated directly by phorbol-esters independent of PKC . It is conceivable that Drosophila PKD is activated in a similar way.
DrosophilaPKD is required for the formation and maintenance of rhabdomeres
Both gain and loss of function of PKD as realized by the overexpression of the presumptive constitutively active PKD-SE isoform and by dsPKD-constructs, respectively, caused a degeneration of the adult retina. Apparently, the fly's retina is highly sensitive towards PKD doses. The phenotypes were quite distinct suggesting that they were not just a consequence of interference with the trafficking of rhodopsin. In this case, we would have expected a degeneration of rhabdomeres in a light and age dependent manner that, in addition, should be amenable to rescue by inhibition of apoptosis [23, 24, 27]. In contrast, the defects caused by the overexpression of the two PKD constructs were only slightly ameliorated by DIAP1. We cannot exclude, however, that the dose of DIAP1 was insufficient for a full rescue. Overall, the retinal degeneration seemed not to be caused primarily by interference with rhodopsin trafficking. Whereas PKD downregulation resembled defects in rhodopsin, the overactivation of PKD caused the elongation and sometimes fragmentation of the rhabdomeres. This phenotype is more reminiscent of mutations in bifocal (bif) and Amphiphysin (Amph) [44, 45]. Bif encodes a presumptive cytoskeletal regulator and associates with actin filaments. Amph is localized to actin-rich membrane domains and is involved in their structural organization. Together, these two genes are required to organize a localized actin cytoskeleton and eventually form the microvilli stacks that build up the rhabdomeres [44, 45]. Based on the similarity of the phenotypes, we propose that manipulation of PKD somehow affects the cytoskeleton and thereby the formation and maintenance of rhabdomeres rather than being involved in rhodopsin trafficking. In fact, rhodopsin itself plays an essential structural role in rhabdomere morphogenesis that involves F-actin and the unconventional myosin ninaC . Accordingly, mutations affecting either rhodopsin, ninaC or components involved in establishment of the microtubule network, i.e. F-actin capping proteins, cause a likewise retinal degeneration [24, 25, 46]. An involvement of PKD in cytoskeletal dynamics would attribute to both, the gain and the loss of function phenotypes. Interestingly, mammalian PKD1 is associated with the F-actin binding proteins cortactin and paxillin in invasive breast cancer cells where it may regulate cell adhesion and motility . In the context of photoreceptor axon guidance, Bif is phosphorylated and thereby regulated by the Ser/Thr kinase misshapen . It is tempting to speculate that PKD might likewise regulate Bif, thereby implementing its influence on the actin cytoskeleton. Further investigations on the relationship of PKD and Bif in Drosophila may help to elucidate the role of human PKDs in the regulation of the cytoskeleton and metastasis.
Our overexpression experiments reveal an involvement of Drosophila PKD in many aspects of development. The effects on vein formation argue for a regulatory input of PKD on one or several signaling pathways, for example dpp-, Notch- or EGFR-pathways. Tissue loss for example in the wing caused by PKD RNAi suggests a role in proliferation and regulation of apoptosis, which is corroborated by respective antibody stainings. A striking degeneration of the adult retina was observed upon downregulation of PKD by RNAi or by overexpression of the presumptive constitutively active PKD-SE isoform. In both cases the formation and maintenance of the rhabdomeres was affected. The observed phenotypes conform to a role of PKD in the regulation of actin dynamics in agreement with similar findings in mammalian cells. Although the eye phenotypes are most likely not a consequence of a disturbed trafficking of rhodopsin, PKD might be involved in the transport of other basolateral cargo for example in extracellular matrix formation of the adult wing. Despite a considerable divergence, human hPKD2 and hPKD3 could largely restore Drosophila PKD RNAi-phenotypes. In summary, our studies are in accordance with a role of PKD in the regulation of cell proliferation and death and hence, the differentiation of various tissues during Drosophila development. Most interestingly, the potential involvement of PKD in the regulation of cytoskeletal dynamics may help to unravel the role of human PKDs in cell motility and metastasis.
Fly strains and crosses
Flies were obtained from the Bloomington stock center, if not mentioned otherwise; information on strains can be found in the flybase. Tissue specific overexpression or RNAi induction was achieved with the Gal4/UAS system [14, 20] using da-Gal, en2.4-Gal4e16E, ey-Gal4 (gift from U. Walldorf), gmr-Gal4, and ptc559-Gal4 as driver lines. Crosses were performed at 18°C, 25°C or 29°C. The following UAS-lines were used: UAS-PKD-WT , UAS-PKD-kd, UAS-PKD-SE, UAS-hPKD2, UAS-hPKD3, UAS-dsPKD (see below), UAS-DIAP1 (gift from A. Müller), UAS-defensin (gift from B. Lemaitre), UAS-PKCi.B4Aand UAS-GFP.
Generation of overexpression constructs and transgenic lines
PKD overexpression constructs fused to GFP were generated as outlined before . Briefly, PKD cDNA was mutated to PKD-kd using the primer pair KWUP 5'-GAG GTG GCC ATC TGG GTG ATC GAC AAA -3' and KWLO 5'- TTT GTC GAT CAC CCA GAT GGC CAC CTC – 3' and to PKD-SE using the primer pair SEUP 5'- ATC GGC GAG AAG GAG TTC CGG CGC GAG GTG GTT GGC ACT -3' and SELO 5'-AGT GCC AAC CAC CTC GCG CCG GAA CTC CTT CTC GCC GAT -3' applying the QuickChange® II XL site-directed mutagenesis kit (Stratagene). Mutant cDNA was shuttled into pEGFP-N1 vector (Clontech) and pUAST vector  as described for PKD-WT . pUAST-hPKD2 and pUAST-hPKD3 were generated from plasmids pEGFP-N1-PKD2 and pEGFP-N1-PKD3 ; cDNA encoding hPKD2 was shuttled as EcoR I/Not I fragment into likewise digested pUAST. cDNA encoding hPKD3 was shuttled as Sac I (blunted by T4 DNA polymerase)/Not I fragment into Eco RI (blunted)/Not I pUAST vector. All constructs were sequence verified. Several transgenic fly lines were generated for each construct and tested for their expression in vivo. Representative lines that had similar expression levels in imaginal discs as well as in Western blots were used for further experiments: PKD-WT10-1, PKD-kd1–2, PKD-kd101-2, PKD-SE112-6, PKD-SE139-2, dsPKD114-5, dsPKD124-4, hPKD2II-2, hPKD2X-2, hPKD3I-3, and hPKD3X-1.
Generation of RNAi construct and transgenic lines
Cloning of a PKD RNAi construct followed the strategy outlined in before . Using the primer pair dsPKDUP 5'-GGA TTC AAA CAG GAG GCG CAG CTG AAG AAC G-3' and dsPKDLO 5'-GGT ACC GGT GTT GGC GCT GCA GCT GAT TAA CTC-3', an 833 nucleotides (nt) spanning C-terminal part of the PKD cDNA (nt 1743 – 2576 starting with A1TG, corresponding to codons 582–836 plus 69 nt from 3' UTR) was cloned into pHIBS. It was shuttled in inverse orientation as Bam HI/Kpn I fragment into likewise opened pUAST vector after destroying an internal Xho I site within the 3' UTR of PKD (position 2537). In a second step, the PKD segment (nt 1743 – 2537) plus the Hairless intron  was cloned in direct orientation as Sal I/Xho I fragment into pUAST opened with Xho I. The final construct was sequence verified. Several transgenic lines were generated according to standard methods. Phenotypes were obtained at 25°C to 29°C in the presence of two or more copies of the dsPKD construct.
The DNA segment cloned in dsPKD showed an overall sequence identity of about 70% to both hPKD2 and hPKD3, respectively . A pairwise alignment of hPKD2 with the Drosophila ds-segment showed a single 24 nucleotide stretch of full identity, however, with lower 5' than 3' stability of the sense strand, making it more likely guide than passenger strand. Moreover, we found 4 stretches of 19 or more nucleotides allowing a single mismatch. In contrast, the hPKD3-dsPKD pair showed a maximum of 11 identical nucleotides and not a single stretch of at least 19 identities allowing a single mismatch. Predictions for potent siRNAs were used to search for optimal pairs, however, not a even one was found that conforms to the signatures of a potent siRNA .
Activity of PKD constructs in human tissue culture
Cell culture and transfection
HEK293T and COS7 cells were grown in RPMI supplemented with 10% fetal calf serum (FCS) in a humified atmosphere containing 5% CO2. HEK293T cells were transfected using TransIT293 reagent (Mirus) according to the manufacturer's instructions. For immunofluorescence, COS7 cells were grown on glass coverslips for 24 hours and transfected with Lipofectamine 2000 reagent (Invitrogen).
HRP transport assay
HEK293T cells were cotransfected with ss-HRP-Flag plasmid and empty pEGFP-N1 vector, pEGFP-N1-PKD-kd and pEGFP-N1-PKD-SE at a ratio of 1: 6.5, respectively. 24 h post-transfection cells were washed with serum-free media and HRP secretion was quantified after 0, 1, 3 and 6 h by incubation of clarified cell supernatant with ECL reagent (Pierce). Measurements were done with a luminometer (Lucy2, Anthos) at 450 nm.
Cells were washed with phosphate buffered saline (PBS), fixed in 4% paraformaldehyde in PBS at room temperature for 10 min and washed with PBS. Coverslips were mounted in Fluoromount G (Southern Biotechnology) and cells were analyzed on a confocal laser scanning microscope (TCS SL, Leica) using 488 nm excitation and a 40.0/1.25 HCX PL APO objective lens. Images were processed with Adobe Photoshop.
Expression analyses in fly tissues
PKD UAS-lines were induced ubiquitously using the da-Gal4 driver. Expression levels were monitored in Western blots loaded with protein extracts of each 100 first instar larvae using anti-GFP antibodies (Santa Cruz Biotechnology). For loading control, the blot was probed simultaneously with anti-gro antibodies (developed by C. Delidakis, obtained from DSHB, University of Iowa, Dept. Biol. Sci., Iowa City, IA 52242, USA). For in situ detection, PKD UAS-lines were crossed with en-Gal4, which drives expression in posterior compartments of imaginal discs. Discs were fixed for 5–10 minutes in 4% paraformaldehyde in phosphate buffered saline (PBS) and mounted in vectashield (Vector lab). To monitor downregulation of PKD by RNAi, the UAS-dsPKD6-4 line was crossed with ptc-Gal4 and imaginal discs of third instar larval offspring processed according to standard methods  using mouse polyclonal anti-PKD antiserum . The posterior compartment of wing discs was stained with monoclonal anti-En/Inv antibodies (Clone 4D9; developed by C. Goodman, obtained from DSHB, University of Iowa, Dept. Biol. Sci., Iowa City, IA 52242, USA). Apoptotic cells were labelled with anti-activated Caspase 3 antibodies (Cell Signaling Technology). Mitotic nuclei were detected with rabbit polyclonal antiserum directed against phosphorylated Histon H3 (Upstate Chemicals). BrdU incorporation was performed as described before  using monoclonal anti-BrdU antibodies (Clone G3G4, developed by S.J. Kaufman, obtained from DSHB). Developing photoreceptor cells were stained in eye imaginal discs using anti-Elav antibodies (Clone 7E8A10, developed by G. Rubin, obtained from DSHB). Secondary antibodies coupled to alkaline phosphatase, fluorescein or Cy3 were purchased from Jackson Laboratories (Dianova). Fluorescent tissue was analyzed by confocal microscopy (BioRad MRC1024 on Zeiss Axioskop). Figures were assembled using Corel Photo Paint and Corel Draw software.
Samples of at least fifty flies of each genotype were inspected with low magnification microscopy (up to 40 fold). For closer examination, wings of ten or more flies were mounted and ten or more eyes were sectioned, respectively. Wings were dried in ethanol, mounted in Euparal (Merck), cleared over night and viewed with a Zeiss Axioskop using Normarski optics or phase contrast. Wing size, cell size and number were determined as described earlier , except that ImageJ software was used for pixel measurements. Fly heads were viewed with a WILD stereomicroscope. Pictures were taken with a Pixera digital camera (Optronics) using the Pixera Viewfinder Version 2.0 software. Sections of adult heads were performed as described before . Figures were compiled using Corel Photo Paint and Corel Draw software.
We are indebted to D. Neumann, A. Kelp and B. van Eijk for their contribution to rescue experiments. We acknowledge the expert technical support by G. Link, M. Ketelhut, T. Stößer and the SFB-Imaging Center. We acknowledge U. Walldorf, B. Lemaitre and the Bloomington stock center for fly lines and DSHB for providing antibodies. We thank members of the two labs for useful discussions and in particular K. Pfizenmaier for helpful suggestions and insights. This work was supported by the German Research Foundation (DFG) through Collaborative Research Center (SFB) 495 and grants to AH (Eliteförderprogramm Landesstiftung Baden-Württemberg and AH 3557/4-1) and DM (DM 1328/8-1), respectively.
- Rykx A, De Kimpe L, Mikhalap S, Vantus T, Seufferlein T, Vandenheede JR, Van Lint J: Protein kinase D: a family affair. FEBS Lett. 2003, 546: 81-86. 10.1016/S0014-5793(03)00487-3.View ArticlePubMedGoogle Scholar
- Wang QJ: PKD at the crossroads of DAG and PKC signaling. Trends Pharmacol Sci. 2006, 27: 317-323. 10.1016/j.tips.2006.04.003.View ArticlePubMedGoogle Scholar
- Brandlin I, Hubner S, Eiseler T, Martinez-Moya M, Horschinek A, Hausser A, Link G, Rupp S, Storz P, Pfizenmaier K, Johannes FJ: Protein kinase C (PKC)eta-mediated PKC mu activation modulates ERK and JNK signal pathways. J Biol Chem. 2002, 277: 6490-6496. 10.1074/jbc.M106083200.View ArticlePubMedGoogle Scholar
- Waldron RT, Rozengurt E: Protein kinase C phosphorylates protein kinase D activation loop Ser744 and Ser748 and releases autoinhibition by the pleckstrin homology domain. J Biol Chem. 2003, 278: 154-163. 10.1074/jbc.M208075200.View ArticlePubMedGoogle Scholar
- Jamora C, Yamanouye N, Van Lint J, Laudenslager J, Vandenheede JR, Faulkner DJ, Malhotra V: Gbetagamma-mediated regulation of Golgi organization is through the direct activation of protein kinase D. Cell. 1999, 98: 59-68. 10.1016/S0092-8674(00)80606-6.View ArticlePubMedGoogle Scholar
- Endo K, Oki E, Biedermann V, Kojima H, Yoshida K, Johannes FJ, Kufe D, Datta R: Proteolytic cleavage and activation of protein kinase C [micro] by caspase-3 in the apoptotic response of cells to 1-beta -D-arabinofuranosylcytosine and other genotoxic agents. J Biol Chem. 2000, 275: 18476-18481. 10.1074/jbc.M002266200.View ArticlePubMedGoogle Scholar
- Vertommen D, Rider M, Ni Y, Waelkens E, Merlevede W, Vandenheede JR, Van Lint J: Regulation of protein kinase D by multisite phosphorylation. Identification of phosphorylation sites by mass spectrometry and characterization by site-directed mutagenesis. J Biol Chem. 2000, 275: 19567-19576. 10.1074/jbc.M001357200.View ArticlePubMedGoogle Scholar
- Feng H, Ren M, Wu SL, Hall DH, Rubin CS: Characterization of a novel protein kinase D: C. elegans DKF-1 is activated by translocation-phosphorylation and regulates movement and growth in vivo. J Biol Chem. 2006, 281 (26): 17801-17814. 10.1074/jbc.M511899200.View ArticlePubMedGoogle Scholar
- Hausser A, Storz P, Martens S, Link G, Toker A, Pfizenmaier K: Protein kinase D regulates vesicular transport by phosphorylating and activating phosphatidylinositol-4 kinase IIIbeta at the Golgi complex. Nat Cell Biol. 2005, 7: 880-886. 10.1038/ncb1289.PubMed CentralView ArticlePubMedGoogle Scholar
- Liljedahl M, Maeda Y, Colanzi A, Ayala I, Van Lint J, Malhotra V: Protein kinase D regulates the fission of cell surface destined transport carriers from the trans-Golgi network. Cell. 2001, 104: 409-420. 10.1016/S0092-8674(01)00228-8.View ArticlePubMedGoogle Scholar
- Yeaman C, Ayala MI, Wright JR, Bard F, Bossard C, Ang A, Maeda Y, Seufferlein T, Mellman I, Nelson WJ, Malhotra V: Protein kinase D regulates basolateral membrane protein exit from trans-Golgi network. Nat Cell Biol. 2004, 6: 106-112. 10.1038/ncb1090.PubMed CentralView ArticlePubMedGoogle Scholar
- Marklund U, Lightfoot K, Cantrell D: Intracellular location and cell context-dependent function of protein kinase D. Immunity. 2003, 19: 491-501. 10.1016/S1074-7613(03)00260-7.View ArticlePubMedGoogle Scholar
- Maier D, Hausser A, Nagel AC, Link G, Kugler SJ, Wech I, Pfizenmaier K, Preiss A: Drosophila protein kinase D is broadly expressed and a fraction localizes to the Golgi compartment. Gene Expr Patterns. 2006Google Scholar
- Brand AH, Perrimon N: Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development. 1993, 118: 401-415.PubMedGoogle Scholar
- Hausser A, Link G, Bamberg L, Burzlaff A, Lutz S, Pfizenmaier K, Johannes FJ: Structural requirements for localization and activation of protein kinase C mu (PKC mu) at the Golgi compartment. J Cell Biol. 2002, 156: 65-74. 10.1083/jcb.200110047.PubMed CentralView ArticlePubMedGoogle Scholar
- Iglesias T, Waldron RT, Rozengurt E: Identification of in vivo phosphorylation sites required for protein kinase D activation. J Biol Chem. 1998, 273: 27662-27667. 10.1074/jbc.273.42.27662.View ArticlePubMedGoogle Scholar
- Storz P, Doppler H, Toker A: Protein kinase Cdelta selectively regulates protein kinase D-dependent activation of NF-kappaB in oxidative stress signaling. Mol Cell Biol. 2004, 24: 2614-2626. 10.1128/MCB.24.7.2614-2626.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Hayashi A, Seki N, Hattori A, Kozuma S, Saito T: PKCnu, a new member of the protein kinase C family, composes a fourth subfamily with PKCmu. Biochim Biophys Acta. 1999, 1450: 99-106. 10.1016/S0167-4889(99)00040-3.View ArticlePubMedGoogle Scholar
- Sturany S, Van Lint J, Muller F, Wilda M, Hameister H, Hocker M, Brey A, Gern U, Vandenheede J, Gress T, Adler G, Seufferlein T: Molecular cloning and characterization of the human protein kinase D2. A novel member of the protein kinase D family of serine threonine kinases. J Biol Chem. 2001, 276: 3310-3318. 10.1074/jbc.M008719200.View ArticlePubMedGoogle Scholar
- Nagel AC, Maier D, Preiss A: Green fluorescent protein as a convenient and versatile marker for studies on functional genomics in Drosophila. Dev Genes Evol. 2002, 212: 93-98. 10.1007/s00427-002-0210-y.View ArticlePubMedGoogle Scholar
- Bard F, Casano L, Mallabiabarrena A, Wallace E, Saito K, Kitayama H, Guizzunti G, Hu Y, Wendler F, Dasgupta R, Perrimon N, Malhotra V: Functional genomics reveals genes involved in protein secretion and Golgi organization. Nature. 2006, 439: 604-607. 10.1038/nature04377.View ArticlePubMedGoogle Scholar
- Stanley H, Botas J, Malhotra V: The mechanism of Golgi segregation during mitosis is cell type-specific. Proc Natl Acad Sci U S A. 1997, 94: 14467-14470. 10.1073/pnas.94.26.14467.PubMed CentralView ArticlePubMedGoogle Scholar
- Satoh AK, O'Tousa JE, Ozaki K, Ready DF: Rab11 mediates post-Golgi trafficking of rhodopsin to the photosensitive apical membrane of Drosophila photoreceptors. Development. 2005, 132: 1487-1497. 10.1242/dev.01704.View ArticlePubMedGoogle Scholar
- Colley NJ, Cassill JA, Baker EK, Zuker CS: Defective intracellular transport is the molecular basis of rhodopsin-dependent dominant retinal degeneration. Proc Natl Acad Sci U S A. 1995, 92: 3070-3074. 10.1073/pnas.92.7.3070.PubMed CentralView ArticlePubMedGoogle Scholar
- Kumar JP, Ready DF: Rhodopsin plays an essential structural role in Drosophila photoreceptor development. Development. 1995, 121: 4359-4370.PubMedGoogle Scholar
- Tzou P, Reichhart JM, Lemaitre B: Constitutive expression of a single antimicrobial peptide can restore wild-type resistance to infection in immunodeficient Drosophila mutants. Proc Natl Acad Sci U S A. 2002, 99: 2152-2157. 10.1073/pnas.042411999.PubMed CentralView ArticlePubMedGoogle Scholar
- Davidson FF, Steller H: Blocking apoptosis prevents blindness in Drosophila retinal degeneration mutants. Nature. 1998, 391: 587-591. 10.1038/35385.View ArticlePubMedGoogle Scholar
- Crozatier M, Glise B, Vincent A: Patterns in evolution: veins of the Drosophila wing. Trends Genet. 2004, 20: 498-505. 10.1016/j.tig.2004.07.013.View ArticlePubMedGoogle Scholar
- de Celis JF: Positioning and differentiation of veins in the Drosophila wing. Int J Dev Biol. 1998, 42: 335-343.PubMedGoogle Scholar
- Sturtevant MA, Bier E: Analysis of the genetic hierarchy guiding wing vein development in Drosophila. Development. 1995, 121: 785-801.PubMedGoogle Scholar
- Johannes B, Preiss A: Wing vein formation in Drosophila melanogaster: hairless is involved in the cross-talk between Notch and EGF signaling pathways. Mech Dev. 2002, 115: 3-14. 10.1016/S0925-4773(02)00083-7.View ArticlePubMedGoogle Scholar
- Rebay I, Fehon RG, Artavanis-Tsakonas S: Specific truncations of Drosophila Notch define dominant activated and dominant negative forms of the receptor. Cell. 1993, 74: 319-329. 10.1016/0092-8674(93)90423-N.View ArticlePubMedGoogle Scholar
- Sturtevant MA, Roark M, Bier E: The Drosophila rhomboid gene mediates the localized formation of wing veins and interacts genetically with components of the EGF-R signaling pathway. Genes Dev. 1993, 7: 961-973. 10.1101/gad.7.6.961.View ArticlePubMedGoogle Scholar
- Urban S, Lee JR, Freeman M: Drosophila rhomboid-1 defines a family of putative intramembrane serine proteases. Cell. 2001, 107: 173-182. 10.1016/S0092-8674(01)00525-6.View ArticlePubMedGoogle Scholar
- Conley CA, Silburn R, Singer MA, Ralston A, Rohwer-Nutter D, Olson DJ, Gelbart W, Blair SS: Crossveinless 2 contains cysteine-rich domains and is required for high levels of BMP-like activity during the formation of the cross veins in Drosophila. Development. 2000, 127: 3947-3959.PubMedGoogle Scholar
- Marcus JM: The development and evolution of crossveins in insect wings. J Anat. 2001, 199: 211-216. 10.1017/S0021878201008226.PubMed CentralView ArticlePubMedGoogle Scholar
- Vilmos P, Sousa-Neves R, Lukacsovich T, Marsh JL: crossveinless defines a new family of Twisted-gastrulation-like modulators of bone morphogenetic protein signalling. EMBO Rep. 2005, 6: 262-267. 10.1038/sj.embor.7400347.PubMed CentralView ArticlePubMedGoogle Scholar
- Kiger JA, Natzle JE, Kimbrell DA, Paddy MR, Kleinhesselink K, Green MM: Tissue remodeling during maturation of the Drosophila wing. Dev Biol. 2007, 301: 178-191. 10.1016/j.ydbio.2006.08.011.PubMed CentralView ArticlePubMedGoogle Scholar
- Kiger JA, Natzle JE, Green MM: Hemocytes are essential for wing maturation in Drosophila melanogaster. Proc Natl Acad Sci U S A. 2001, 98: 10190-10195. 10.1073/pnas.181338998.PubMed CentralView ArticlePubMedGoogle Scholar
- Oster H, Abraham D, Leitges M: Expression of the protein kinase D (PKD) family during mouse embryogenesis. Gene Expr Patterns. 2006, 6: 400-408. 10.1016/j.modgep.2005.09.006.View ArticlePubMedGoogle Scholar
- Reynolds A, Leake D, Boese Q, Scaringe S, Marshall WS, Khvorova A: Rational siRNA design for RNA interference. Nat Biotechnol. 2004, 22: 326-330. 10.1038/nbt936.View ArticlePubMedGoogle Scholar
- Auer A, von Blume J, Sturany S, von Wichert G, Van Lint J, Vandenheede J, Adler G, Seufferlein T: Role of the regulatory domain of protein kinase D2 in phorbol ester binding, catalytic activity, and nucleocytoplasmic shuttling. Mol Biol Cell. 2005, 16: 4375-4385. 10.1091/mbc.E05-03-0251.PubMed CentralView ArticlePubMedGoogle Scholar
- Rey O, Sinnett-Smith J, Zhukova E, Rozengurt E: Regulated nucleocytoplasmic transport of protein kinase D in response to G protein-coupled receptor activation. J Biol Chem. 2001, 276: 49228-49235. 10.1074/jbc.M109395200.View ArticlePubMedGoogle Scholar
- Bahri SM, Yang X, Chia W: The Drosophila bifocal gene encodes a novel protein which colocalizes with actin and is necessary for photoreceptor morphogenesis. Mol Cell Biol. 1997, 17: 5521-5529.PubMed CentralView ArticlePubMedGoogle Scholar
- Zelhof AC, Hardy RW: WASp is required for the correct temporal morphogenesis of rhabdomere microvilli. J Cell Biol. 2004, 164: 417-426. 10.1083/jcb.200307048.PubMed CentralView ArticlePubMedGoogle Scholar
- Delalle I, Pfleger CM, Buff E, Lueras P, Hariharan IK: Mutations in the Drosophila orthologs of the F-actin capping protein alpha- and beta-subunits cause actin accumulation and subsequent retinal degeneration. Genetics. 2005, 171: 1757-1765. 10.1534/genetics.105.049213.PubMed CentralView ArticlePubMedGoogle Scholar
- Bowden ET, Barth M, Thomas D, Glazer RI, Mueller SC: An invasion-related complex of cortactin, paxillin and PKCmu associates with invadopodia at sites of extracellular matrix degradation. Oncogene. 1999, 18: 4440-4449. 10.1038/sj.onc.1202827.View ArticlePubMedGoogle Scholar
- Ruan W, Long H, Vuong DH, Rao Y: Bifocal is a downstream target of the Ste20-like serine/threonine kinase misshapen in regulating photoreceptor growth cone targeting in Drosophila. Neuron. 2002, 36: 831-842. 10.1016/S0896-6273(02)01027-9.View ArticlePubMedGoogle Scholar
- Maier D, Nagel AC, Preiss A: Two isoforms of the Notch antagonist Hairless are produced by differential translation initiation. Proc Natl Acad Sci U S A. 2002, 99: 15480-15485. 10.1073/pnas.242596699.PubMed CentralView ArticlePubMedGoogle Scholar
- de Nooij JC, Letendre MA, Hariharan IK: A cyclin-dependent kinase inhibitor, Dacapo, is necessary for timely exit from the cell cycle during Drosophila embryogenesis. Cell. 1996, 87: 1237-1247. 10.1016/S0092-8674(00)81819-X.View ArticlePubMedGoogle Scholar
- Rulifson EJ, Kim SK, Nusse R: Ablation of insulin-producing neurons in flies: growth and diabetic phenotypes. Science. 2002, 296: 1118-1120. 10.1126/science.1070058.View ArticlePubMedGoogle Scholar
- Basler K, Hafen E: Control of photoreceptor cell fate by the sevenless protein requires a functional tyrosine kinase domain. Cell. 1988, 54: 299-311. 10.1016/0092-8674(88)90193-6.View ArticlePubMedGoogle Scholar
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