Control of cell migration in the development of the posterior lateral line: antagonistic interactions between the chemokine receptors CXCR4 and CXCR7/RDC1
© Dambly-Chaudière et al; licensee BioMed Central Ltd. 2007
Received: 23 November 2006
Accepted: 29 March 2007
Published: 29 March 2007
The formation of the posterior lateral line of teleosts depends on the migration of a primordium that originates near the otic vesicle and moves to the tip of the tail. Groups of cells at the trailing edge of the primordium slow down at regular intervals and eventually settle to differentiate as sense organs. The migration of the primordium is driven by the chemokine SDF1 and by its receptor CXCR4, encoded respectively by the genes sdf1a and cxcr4b. cxcr4b is expressed in the migrating cells and is down-regulated in the trailing cells of the primordium. sdf1a is expressed along the path of migration. There is no evidence for a gradient of sdf1a expression, however, and the origin of the directionality of migration is not known.
Here we document the expression of a second chemokine receptor gene, cxcr7, in the migrating primordium. We show that cxcr7 is highly expressed in the trailing cells of the primordium but not at all in the leading cells, a pattern that is complementary to that of cxcr4b. Even though cxcr7 is not expressed in the cells that lead primordium migration, its inactivation results in impaired migration. The phenotypes of cxcr4b, cxcr7 double morphant embryos suggest, however, that CXCR7 does not contribute to the migratory capabilities of primordium cells. We also show that, in the absence of cxcr4b, expression of cxcr7 becomes ubiquitous in the stalled primordium.
Our observations suggest that CXCR7 is required to provide directionality to the migration. We propose that directionality is imposed on the primordium as soon as it comes in contact with the stripe of SDF1, and is maintained throughout migration by a negative interaction between the two receptors.
Directed cell migration is involved in many aspects of development including the establishment of the embryonic body plan, organogenesis and organ function. It also plays a role in several pathological processes, notably the spread of tumour cells and formation of metastases. Identification of the molecules governing cell migration is therefore of major importance. Most work on cell migration relies on in vitro systems where migration is relatively easy to monitor and quantify. This has led to substantial progress in understanding the cell biology of migration as well as the many receptor molecules and signaling cascades involved. Migration is crucially dependent on the cell environment, however, and ideally one would like to study its control in a system where migration can be visualized in vivo and in real time.
The lateral-line system of the zebrafish has emerged recently as a useful model for studying the process of long-distance cell migration and for unraveling its genetic control . The lateral-line is a mechanosensory system used by fish to detect water movements and plays an important role in a variety of behaviours . It comprises discrete sense organs, the neuromasts, arranged on the body surface in species-specific patterns. The posterior lateral line (PLL), which extends on the trunk and tail, comprises at the end of embryogenesis a line of five neuromasts regularly spaced along the trunk and tail, and a cluster of two-three terminal neuromasts at the tip of the tail . This pattern is widely conserved among teleost embryos .
All neuromasts of the PLL originate from a sensory placode that forms just posterior to the otic vesicle [5, 6]. A group of about 100 cells delaminate from the placode to form a migrating primordium that moves all the way to the tip of the tail at a constant speed of 1.7 somite/h . The journey lasts 20 h, from 20 to 40 hpf, and the migrating primordium deposits in its wake five groups of cells that will become the neuromasts L1 – L5. Migrating cells keep their relative positions within the migrating primordium, and each deposition results from a progressive slowing down of a group of around 20 cells at the trailing edge [7, 8]. Once these 20 cells have settled down, they differentiate as hair cells and support cells to form a neuromast. Neuromasts are connected by a thin stripe of interneuromastic cells that also arise from the migrating primordium; these cells will later form intercalary neuromasts [9, 10]. Upon reaching the tip of the tail the primordium fragments in 2–3 groups that will form the terminal neuromasts .
The primordium is guided along a trail of cells that express the chemokine SDF1, and its migration depends on the partner of SDF1, the chemokine receptor CXCR4 [11, 12]. One of the two genes coding for this receptor, cxcr4b, is expressed in the migrating cells and is down-regulated in the cells at the trailing edge of the primordium . The inactivation of sdf1a in morphant embryos, or of cxcr4b in mutant or morphant embryos, results in an arrest of migration [11, 12]. A similar effect of cxcr4b inactivation has been observed in a mutant line of the more derived fish Oryzias latipes (medaka) . Medaka belongs to the neoteleost lineage, while the zebrafish belongs to the more primitive ostariophysian lineage. This suggests that not only the early pattern of the PLL but also the underlying mechanism is highly conserved among teleosts.
In an attempt to discover other elements that contribute to the control of migration we have examined other genes that display heterogeneous patterns of expression within the migrating primordium. Here we report the description of another chemokine receptor, CXCR7. Although long considered an orphan receptor, CXCR7 has recently been shown to recognize SDF1  and possibly other ligands as well . We show that CXCR7 plays an essential role for primordium migration in spite of not being expressed in the vast majority of the migrating cells, and we propose that it is required to provide migration directionality.
Identification of cxcr7, a gene potentially involved in the control of PLL primordium migration
SDF1 has been shown to bind to the N-terminal, extracytoplasmic domain of CXCR4 . A small stretch of 6 aminoacids is conserved between human and fish CXCR4, of which 2 (D20 Y21) have been shown in Homo to be important for the binding of HIV. Besides this short motif, however, there is very little sequence conservation between the N-terminal domains of human and fish CXCR4. There is even less N-terminal conservation between fish CXCR4 and CXCR7, or between fish and human CXCR7 (Fig. 1). The remarkable lack of conservation of the SDF1-binding domain suggests that the recognition of SDF1 is not based on conventional stereochemical matching. This conclusion is fully consistent with the observation that a D-amino-acid version of SDF1 binds to the human CXCR4 receptor as well or even better than the normal L-version .
Contrary to the poor conservation of the N-terminal extracellular region, the predicted C-terminal intracellular domain of human and fish CXCR7 are 73% identical. The level of identity is somewhat lower between the human and fish CXCR4 (55%). Interestingly, however, there is essentially no conservation between the C-terminals of the two receptors (an amazingly low 6% in either fish or human), strongly suggesting that CXCR4 and CXCR7 act through different cytoplasmic effectors and play different roles in the control of migration.
cxcr7expression in the PLL primordium
Shortly after primI has reached the tip of the tail and formed the terminal neuromasts, a second primordium arises. This primordium, primII, migrates along the same path as primI and deposits a second wave of about 5 neuromasts [21, 22]. The migration of primII is slower than that of primI, and the neuromasts of the second wave are more closely packed. Their polarity is orthogonal to that of the primary neuromasts deposited by primI . We examined the expression pattern of cxcr7 at 2 days, when primII has reached somite 7 on average. We observed no cxcr7 expression in primII (Fig. 2E).
Besides the PLL, cxcr7 is expressed in other discrete regions, notably in parts of the hindbrain, midbrain, forebrain (diencephalon), nose, eye, and kidneys (not shown). In most places the pattern of expression of cxcr7 appears highly dynamical.
Expression of cxcr7 and cxcr4during primordium migration
The gene cxcr7 is expressed in the trailing part of the primordium, that is, in the cells that are about to be deposited (Fig. 3A). It might be, therefore, that cxcr7 expression is lost in the migrating primordium just after deposition. We examined the transitional pattern when the cells with a strong expression of cxcr7 are slowing down. We observed in all cases that the cells at the new trailing edge weakly express cxcr7 (Fig. 3B) and that this weak expression quickly increases after deposition (Fig. 3A). Thus the expression of cxcr7 in trailing cells is not re-initiated after each deposition, but amplified to maintain a dynamical asymmetry within the primordium.
The pattern of expression of cxcr7 in the migrating primordium is almost complementary to the pattern reported for cxcr4b . The gene cxcr4b codes for the chemokine receptor CXCR4 which plays an essential role in the migration of the PLL primordium [11, 12]. cxcr4b is strongly expressed in the leading two thirds of the primordium and its expression is down-regulated in the trailing third (Fig. 3C). In order to better define the relation between the two patterns we did a double in situ hybridization experiment (Fig. 3D). We observed that the domains of expression of cxcr7 and of cxcr4b are largely but not completely exclusive, as there is some overlap of expression in the trailing cells. Thus a high level of expression of cxcr4b seems to exclude the expression of cxcr7, but a high level of cxcr7 expression does not preclude the expression of cxcr4b.
Early expression of cxcr7, cxcr4b, and sdf1a
A comparison of the profiles of cxcr7 and cxcr4b around 22hpf suggests that cxcr7 is up-regulated and cxcr4b is down-regulated in the prospective trailing cells at the onset of migration. We cannot tell whether the up-regulation of cxcr7 and down-regulation of cxcr4b are exactly simultaneous, however, as there is some variability among embryos (e.g. the primordium is almost identical in shape and position in Figs. 5C and 5D, yet down-regulation of cxcr4b is evident in D but not in C) and double in situ hybridization is not as sensitive as single in situ in our hands.
Inactivation of cxcr7alters the pattern of neuromasts
Numbers of neuromasts in morphant embryos
cxcr7-MO + cxcr4b-MO1
cxcr7-MO + cxcr4b-MO2
Position of neuromasts in morphant embryos
cxcr7-MO + cxcr4b-MO2
In wild type embryos, primI reaches the tip of the tail at about 40 hpf. There it fragments to form 2–3 closely apposed terminal neuromasts . In morphant embryos at 48 hpf, the primordium is still visible in 90% of the cases, either at a very anterior position in the embryos where no or one neuromast has formed (Fig. 7D) or close to the last deposited neuromast in embryos where 2–5 neuromasts have formed. We occasionally observed 2 or 3 incompletely separated neuromasts (Fig. 7C), a pattern that is reminiscent of the fragmentation that takes place when primI has reached the tip of the tail in wild type embryos.
Inactivation of cxcr7affects primordium migration
The distribution of neuromasts along the antero-posterior axis is clearly affected in cxcr7 morphant embryos (Fig. 7). The reduced number and abnormal distribution of neuromasts suggest a defect in migration of the PLL primordium. Since the development of other structures (pectoral fins, eyes, ear and anterior lateral line) appears completely normal in cxcr7 morphants, the defect in migration does not result from a general impairment of development.
In order to confirm that migration is defective in the morphants we followed the course of the primordium under Nomarski optics. We examined 12 morphant embryos every 3 hours between 24 hpf and 36 hpf and determined the position of the leading edge of primI. We also determined the pattern of neuromasts at 48 hpf after alkaline phosphatase labelling. We observed that either the primordium does not migrate and extends no further than somite 2 at most (3 cases), or that it migrates at a reduced speed (9 cases). The speed varied between 0.2 somite and 0.7 somite per hour depending on the embryo, with an average of 0.4 ± 0.17 somite per hour. The speed in the wild type is 1.5 – 1.7 somite/hour. In the 9 embryos where migration was slowed down, the position reached by the primordium at 48 hpf was at most two somites beyond the position that the primordium occupied at 36 hpf, suggesting that migration stopped a few hours after 36 hpf, at about the time when migration stops in the wild type (40 hpf).
Comparison of cxcr4b-MO and cxcr7-MO phenotypes
The gene cxcr4b is essential for proper migration of the primordium. Its pattern of expression fits well with this role, as it is highly expressed in the migrating cells of the primordium and less so in the trailing cells which are beginning to slow down. We have shown that the gene cxcr7 is also required for proper migration, yet its pattern of expression is opposite to that of cxcr4b as it is highly expressed in the cells that are being deposited, and not at all in the actively migrating cells.
In order to determine whether there is some interaction between cxcr4b and cxcr7, we first compared the phenotypes of cxcr4b-MO, of cxcr7-MO and of double cxcr4b-MO, cxcr7-MO embryos at 48 hpf. We used two different cxcr4b morpholinos, as discussed in Methods, one with a low survival rate, low penetrance and very high expressivity (cxcr4b-MO1, ) and one with a much higher survival rate and penetrance but a lower expressivity (cxcr4b-MO2, ). Since the phenotypes produced by the two morpholinos are somewhat different, we will discuss the results separately.
The phenotype of embryos injected with cxcr4b-MO1 is very similar to the strongest phenotype of cxcr7-MO, with one or two neuromasts around somite 1 (Fig. 7E and Table 1). We did not observe the intermediate phenotypes that are often present in cxcr7 morphants, with 1–4 neuromasts extending between somites 2 and 15 approximately. Interestingly, the severity of the cxcr4b-MO1 phenotype is largely relieved by the simultaneous inactivation of cxcr7 in double morphant embryos. In this case, up to 30% of the affected embryos show intermediate phenotypes that are typical of the cxcr7 morphants (Table 1). In many affected embryos the stalled primordium is still visible at 48 hpf after alkaline phosphatase labelling. We observed that the primordium reaches the posterior half of the body in 38% of the double morphant embryos (N = 32), very similar to the proportion in cxcr7-MO embryos (33%, N = 45). The primordium never extends beyond somite 5 in embryos that are injected with cxcr4b-MO alone. It appears, therefore, that the expression of cxcr7 aggravates the effect of cxcr4b deprivation. We conclude that CXCR7 may have an antagonistic role to that of CXCR4 in the primordium, consistent with their complementary patterns of expression.
The phenotype of embryos injected with cxcr4b-MO2 is milder than that of cxcr4b-MO1 morphants and resembles that of cxcr7 morphants (Table 1). The phenotype of the double cxcr4b, cxcr7 morphant is very similar to that of single cxcr4b-MO2 and cxcr7-MO injected embryos. Intriguingly, however, we had the impression that the pattern of neuromasts in the cxcr4b-MO2 morphant is more irregular that in either the cxcr7 or the double morphant. We quantified this impression by determining the standard deviation of the positions of L1 to L4 in all cases where only four neuromasts were present (Table 2). The results show that the pattern is substantially more irregular in cxcr4b-MO2 embryos than in either the cxcr7 or the double morphant, suggesting that the expression of cxcr7 in the presence of reduced levels of CXCR4 makes migration more erratic.
Expression of cxcr4b in cxcr7-MO embryos
The non-migrating primordium usually assumes a round shape (, Fig. 9A) and does not show any clear heterogeneity or asymmetry in the expression of cxcr4b, suggesting that CXCR7 plays a role in establishing or maintaining the asymmetry of cxcr4b expression. In cases where the primordium shows abortive migration and reaches somites 2–5, the expression of cxcr4b is lower in the trailing cells than in other cells (Fig. 9B, arrow), suggesting that the asymmetry in cxcr4b expression does not entirely depend on the presence of CXCR7 in the trailing region. Even in this case, however, the asymmetry in cxcr4b is not as pronounced as in a normally migrating primordium (compare Fig. 9B and 3C). We conclude that the presence of CXCR7 in the trailing cells contributes to the down-regulation of cxcr4b. This conclusion must remain tentative because the expression of cxcr4b is dynamic: in normal conditions the expression is more homogeneous after deposition and more asymmetrical prior to deposition, complementary to the pattern of expression of cxcr7 (Fig. 3A, 3B).
Expression of cxcr7 in cxcr4b-MO and in sdf1a-MO embryos
Given the complementarity in the patterns of expression of cxcr7 and cxcr4b we also examined the expression of cxcr7 in the non-migrating primordium of cxcr4b morphants (Fig. 9C, 9E). The outlines of non-migrating primordia are not as distinct under Nomarski optics as those of normal primordia but it appears clearly that most or all primordium cells express cxcr7 in morphant embryos (Fig. 9C; in this embryo part of the primordium has reached somite 2–3 and extends over the yolk, arrow). In another revealing case (Fig. 9E), one half of the primordium has remained paralysed around somite 1, while the other half has migrated (although at a reduced pace), suggesting that there was enough residual expression of cxcr4b in those cells to ensure some migration. All cells of the stalled group express cxcr7. Within the migrating group cxcr7 is expressed exclusively in the trailing cells. We conclude that the expression of cxcr4b is required to confine cxcr7 expression to the trailing region of the primordium.
If repression of cxcr7 in the leading region of the primordium depends on the activity of CXCR4, one would expect to observe ubiquitous expression of cxcr7 not only in the absence of CXCR4, but also when CXCR4 activation is prevented by the absence of its ligand. We examined the expression of cxcr7 in sdf1a-MO embryos . We observed a ubiquitous expression of cxcr7 in stalled primordia (Fig. 9D) much as in the case of cxcr4b morphants. We also saw two cases of split primordium similar to the case shown Fig. 9E.
Effect of cxcr7inactivation on the formation of the secondary lateral line
We examined the effect of cxcr7 inactivation on the migration of primII at 2 and 6 days. At 2 days, primII is located between somites 4 and 7 in wild type embryos. In cxcr7-MO embryos of the same age, we observed that migration of primII is affected and that the severity of this effect is correlated with the severity of the effect on primI migration: no migration when primI is immobilized in the 0s-5s region, migration in 20% of the cases (2/10) where primI is stalled between 10s-15s, in 35% of the cases (5/14) when primI is found between 16s-25s and in 100% of the cases (N = 10) when primI migrates normally.
The primordium of the dorsal line originates together with primII and the two primordia split at about 36 hpf . The dorsal primordium deposits the first neuromast of the dorsal line, D1, shortly thereafter. We observed that in cxcr7 morphants neuromast D1 is present in all cases, suggesting that the secondary primordium forms normally (Fig. 7D). The same result is observed in cxcr4b morphants (Fig. 7E).
We verified this result in 6 days-old larvae where primII has deposited 3–4 neuromasts and the dorsal line also comprises 2–3 neuromasts. Secondary PLL neuromasts can be distinguished from primary neuromasts by their polarization which is revealed by anisotropic alkaline phosphatase labelling. Among 42 severely affected cxcr7-MO embryos with no primary neuromast or one neuromast on somite 1, only 2 had formed a secondary neuromast, but all of them had developed a normal dorsal line (not shown), supporting the idea that the inactivation of cxcr7 affects specifically the migration of primII.
Since cxcr7 is not detectably expressed in primII, the easiest explanation for the lack of primII migration in morphant embryos is that primII relies on a trail left by primI (possibly the nerve, or the interneuromastic cells) such that if primI does not migrate neither can primII. The effect of cxcr7 inactivation on primII migration would therefore be indirect. We cannot, however, exclude the possibility that cxcr7 is transcribed in primII at such a low level that its expression would escape detection by in situ hybridization.
Migration as a collective process
The zebrafish lateral line is emerging as an attractive system to study programmed cell migration. A number of studies have conclusively demonstrated that in this system migration depends on the interaction between the chemokine SDF1, which labels the path of migration, and its receptor CXCR4, which is present on the migrating cells [8, 11, 12]. SDF1/CXCR4 interactions also underly other long-range migration events such as the movement of germ cells both in fish [25, 26] and in mouse , the migration of facial motoneurons in fish  and the movement of tumor cells in the formation of metastases .
Much has been learned about the implication of the SDF1/CXCR4 system in cell migration in the immune system, where cells seem to behave independently of each other. In the case of the PLL, however, cells move as a disciplined cohort and act in a coordinated manner. They keep their relative positions during migration and the cells that are deposited are always the trailing cells of the primordium [7, 8]. In the case of the germ cells, cells remain in contact during their migration although they do not show the stable organization of the primordium cells . In the case of cancer cells, collective or cohort migration has also been documented .
cxcr7and primordium migration
The gene cxcr4b is expressed in all cells of the primordium but its level of expression is lower in the trailing cells, consistent with the fact that those cells will soon slow down and stop migrating. Thus the pattern of expression of cxcr4b fits perfectly with an active role in cell migration. In this paper we describe the expression of the gene that encodes another chemokine receptor, CXCR7. The gene cxcr7 is expressed in the primordium in a pattern that is complementary to that of cxcr4b. Thus cxcr7 is maximally expressed in the cells that will be deposited next, and not at all in the actively migrating cells of the leading half of the primordium. It came as a surprise, therefore, to find that the inactivation of cxcr7 blocks migration much as the inactivation of cxcr4b. We heard from Darren Gilmour, at a recent meeting (15–18 March 2007, Minerve, France) that he has obtained very similar results about the expression and inactivation of cxcr7.
A cue to the function of cxcr7 comes from the analysis of the simultaneous inactivation of cxcr7 and of cxcr4b, as compared to the inactivation of cxcr4b alone. The phenotype of cxcr4b morphants, where cxcr7 is active, is substantially stronger than the phenotype of the double morphant, where cxcr7 is not active. We conclude that in conditions of reduced cxcr4b expression, the expression of cxcr7 has a negative effect on the residual migration of the primordium, consistent with its expression in the cells that are slowing down in wild type embryos.
A second cue to the function of cxcr7 comes from the observation that the inactivation of cxcr4b results in a deregulation of cxcr7, which becomes expressed in most or all cells of the primordium instead of being confined to its anteriormost (trailing) region. The down-regulation of cxcr7 by SDF1/CXCR4 in wild type embryos is consistent with the idea that the presence of CXCR7 in the leading cells would be detrimental for migration. Deregulation of cxcr7 in cxcr4b-MO embryos probably contributes to the aggravation of phenotype observed in cxcr4b-MO1 embryos vs the double cxcr4b, cxcr7 morphants.
How could a receptor that has a negative effect on migration be indispensable for migration? One obvious possibility is that CXCR7 is involved in defining the directionality of migration. Thus cxcr7 inactivation would not impair migration per se, but would make it impossible for the primordium cells to move coherently in one direction, thereby resulting in stalling of the direction-less primordium.
The PLL primordium migrates consistently from anterior to posterior even though the expression of sdf1 appears constant along the myoseptum. This suggest that the primordium has an intrinsic polarity, with a "plus" end at its leading edge and a "minus" end at the trailing edge. The idea that the primordium is intrinsically polarized is supported by experiments showing that a primordium confronted to an interruption in the SDF1 trail will sometimes make a U-turn and follow the trail of SDF1 in the opposite direction, towards the head . Furthermore, it has been shown that a few wild-type cells at the leading edge are sufficient to rescue the migration of the entire primordium when cxcr4b is inactivated . These results demonstrate that an intrinsic asymmetry in cxcr4b expression underlies the directionality of primordium migration. Our results suggest that this intrinsic asymmetry depends at least in part on the localized expression of cxcr7 in the trailing region and/or on its absence in the leading region.
An antagonistic effect of CXCR7 on CXCR4 activation could be based on the high affinity of CXCR7 for SDF1, ten times higher than the affinity of CXCR4 for the same ligand . The efficient binding of SDF1 to CXCR7 would lead to a masking or sequestering of SDF1 in the trailing region of the primordium, thereby making it unavailable to CXCR4. Furthermore data obtained in other systems suggest that the activation of CXCR4 may positively control the expression of the cxcr4 gene. The activation of CXCR4 by SDF1 promotes the formation of NFκB , which itself can induce the expression of cxcr4 . We have evidence that this positive feedback loop is active in the PLL primordium (J. Torgersen, CDC, NC and AG, in preparation). The feedback loop would be interrupted when SDF1 is sequestered or masked, thereby leading indirectly to a reduction in cxcr4b expression. Thus an antagonistic effect of CXCR7 on CXCR4 activity may be achieved at two levels: first by sequestering its ligand SDF1, second by preventing the self-activation of cxcr4b. We cannot rule out, of course, that in addition to an inhibition of CXCR4 signalling, CXCR7 activation by SDF1 also has a more direct effect on migration directionality.
In morpholino conditions, reduced levels of cxcr4b expression may result in fluctuations in the cellular concentration of CXCR4, with some cells having a higher or lower concentration relative to their companions. The cells with a higher residual level of CXCR4 may then take the lead and the cells with a lower level may end up in the trailing region , thereby re-establishing some level of polarity and allowing some migration even if cxcr7 is not expressed. This would explain why, depending on the strength of cxcr4b morpholino inactivation, the expression of cxcr7 may either prevent migration altogether (cxcr4b-MO1), or simply make it more erratic (cxcr4b-MO2).
Origin of primordium polarization
How could the anisotropy in the expression of cxcr4b and cxcr7 be initiated? We have observed that cxcr4b is expressed before the onset of migration, while cxcr7 is expressed later on. As the primordium splits from the ganglion and elongates, its most posterior cells come in contact with the stripe of SDF1 (which extends along the horizontal myoseptyum but not into the head). Activation of CXCR4 by SDF1 will induce migration of these cells along the SDF1 track, bringing the next cells in contact with SDF1. The migration of more and more cells along the myoseptum will lead to a progressive depletion of SDF1 through internalization of the ligand-receptor complex. Thus the last cells to come in will have a reduced level of CXCR4 activation, thereby allowing cxcr7 to become expressed. This would establish an early anisotropy of the primordium which would then be maintained due to the negative effect of cxcr4b on cxcr7 expression in the leading cells, and to the reciprocal negative effect of CXCR7 on CXCR4 function (through masking of SDF1) in the trailing cells.
Such a stable anisotropy would explain why, when a primordium turns back due to an interruption in the trail of SDF1, the cells do not simply go the other way around but the entire primordium doubles upon itself in a spectacular U-turn, such that its leading region will remain at the leading edge . The fact that this turn is observed in only one tenth of the cases is consistent with the idea that the guiding trail has been at least partly depleted of SDF1 through binding and internalization of the ligand by both CXCR4 and CXCR7.
The migration of primordium cells as an organized cohort  may thus be in itself sufficient to generate its own directionality, since the concentration of SDF1 available to trailing cells will necessarily be lower than that available to leading cells (due to ligand/receptor internalization). CXCR7 would contribute to the control of primordium migration by reinforcing and stabilizing this intrinsic directionality, thereby allowing the fast and reproducible journey that is the basis for PLL development.
We propose that the directional migration of the PLL primordium is determined by an intrinsic asymmetry due to the reciprocal distribution of two chemokine receptors that recognize the same ligand, chemokine SDF1: CXCR4 at the leading edge of the migrating primordium, and CXCR7 at its trailing edge. The interplay between the two receptors ensures that a constant distribution of SDF1 along the pathway is translated as a graded distribution of activated CXCR4 along the primordium, forcing primordium cells to move in the direction of higher SDF1/CXCR4 signalling, that is, in the direction of the leading cells and away from the trailing cells. The reciprocal expression of the two receptor genes is maintained through antagonistic interactions, and may originate automatically at the onset of migration due to the presence of the SDF1 stripe at one side of the newly born primordium.
Zebrafish (Danio rerio) were obtained from Singapour through a local company, Antinea, and maintained in standard conditions . Embryos were obtained from pairs of adult fish by natural spawning and raised at 28.5°C in tank water. Ages are expressed as hours post fertilization (hpf).
Identification of cxcr7
The gene cxcr7 was initially identified as an EST (cb900) and selected on the basis of its expression pattern . This EST corresponds to gene si:dkey-96h14.2. Sequence alignment with mammalian genomes (Homo sapiens, Mus Musculus and Rattus norvegicus) revealed that this gene codes for the fish homolog of the chemokine receptor CXCR7.
Morpholino knockdown experiments
Morpholinos oligonucleotides (Gene Tools, USA) were dissolved at 1.25 mM in 0.2 mM KCl and injected at the one-cell stage. This concentration gave the best combination of survival and phenotype. When 2 morpholinos were injected simultaneously, the solution contained each morpholino at a concentration of 1.25 mM. The antisense Morpholino sequences were designed to inhibit the translation of cxcr7 or cxcr4b mRNA. The MO-cxcr7 sequence is: 5'TCATTCACGTTCACACTCATCTTGG-3'. The control morpholino had the following mismatches (underlined) : 5'TCATACACCTTGACACA CATCTAGG-3'.
In the case of cxcr4b, two Morpholinos sequences were used: MO1-cxcr4b (5'- ATGATGCTATCGTAAAATTCCATTT-3', ) and MO2-cxcr4b (5'-AAATGATGCTATCGTAAAATTCCAT-3', ). The bold CAT corresponds to the ATG translation start codon. As noted previously , MO1-cxcr4b morphants have a low penetrance (about 40% of injected embryos show an abnormal phenotype) but a high expressivity (the abnormal embryos show an extreme phenotype). Inactivation of sdf1a was done as described previously .
In Situ Hybridization
20 to 35 hpf embryos were manually dechorionated, fixed in PBS-4%PFA for 2 hr at room temperature, rinsed in PBS and in 100% methanol and kept at -20°C. They were then processed for in situ hybridisation as described in [7, 36].
Neuromast labeling with alkaline phosphatase
48 hpf embryos were dechorionated, fixed in PBS-4%PFA for 2 hr at room temperature, rinsed in PBS and, if necessary, kept at 4° in PBS for up to a week. They were then processed for alkaline phosphatase labeling as described in .
This work was supported by ANR (Agence Nationale pour la Recherche, France) and by ARC (Association pour la Recherche sur le Cancer, France). We thank Mireille Rossel for discussions and help with sequence comparisons, Fanny Estermann for early observations on the cxcr7 morphant phenotype, David Raible for discussion and comments on the manuscript, and Darren Gilmour for communicating results prior to publication.
- Ghysen A, Dambly-Chaudière C: Development of the zebrafish lateral line. Curr Op Neurobiol. 2004, 14: 67-73. 10.1016/j.conb.2004.01.012.View ArticlePubMedGoogle Scholar
- The Mechanosensory Lateral Line, Neurobiology and Evolution. Edited by: Coombs S, Görner P, Münz H. 1989, New York: Springer-VerlagGoogle Scholar
- Metcalfe W: Sensory neuron growth cones comigrate with posterior lateral line primodial cells in Zebrafish. J Comp Neurol. 1985, 238: 218-224. 10.1002/cne.902380208.View ArticlePubMedGoogle Scholar
- Pichon F, Ghysen A: Evolution of posterior lateral line development in fish and amphibians. Evol Dev. 2004, 3: 187-193. 10.1111/j.1525-142X.2004.04024.x.View ArticleGoogle Scholar
- Harrison RG: Experimentelle Untersuchungen über die Entwicklung der Sinnesorgane der Seitenlinie bie den Amphibien. Arch Mikrosk Anat. 1904, 63: 35-49.View ArticleGoogle Scholar
- Stone L: Experiments on the development of lateral-line sense organs in amphibians observed in living and vital-stained preparations. J Comp Neurol. 1933, 57: 507-540. 10.1002/cne.900570307.View ArticleGoogle Scholar
- Gompel N, Cubedo N, Thisse C, Thisse B, Dambly-Chaudière C, Ghysen A: Pattern formation in the lateral line of zebrafish. Mech Dev. 2001, 105: 69-77. 10.1016/S0925-4773(01)00382-3.View ArticlePubMedGoogle Scholar
- Haas P, Gilmour D: Chemokine signaling mediates self-organizing tissue migration in the zebrafish lateral line. Dev Cell. 2006, 10: 673-80. 10.1016/j.devcel.2006.02.019.View ArticlePubMedGoogle Scholar
- Grant KA, Raible DW, Piotrowski T: Regulation of latent sensory hair cell precursors by glia in the zebrafish lateral line. Neuron. 2005, 45: 69-80. 10.1016/j.neuron.2004.12.020.View ArticlePubMedGoogle Scholar
- Lopez-Schier H, Hudspeth AJ: Supernumerary neuromasts in the posterior lateral line of zebrafish lacking peripheral glia. Proc Natl Acad Sci USA. 2005, 102: 1496-501. 10.1073/pnas.0409361102.PubMed CentralView ArticlePubMedGoogle Scholar
- David N, Sapède D, St-Etienne L, Thisse C, Thisse B, Dambly-Chaudière C, Rosa F, Ghysen A: Molecular basis of cell migration in the fish lateral line: role of the chemokine receptor CXCR4 and of its ligand, SDF1. Proc Natl Acad Sci USA. 2002, 99: 16297-16302. 10.1073/pnas.252339399.PubMed CentralView ArticlePubMedGoogle Scholar
- Li Q, Shirabe K, Kuwada JY: Chemokine signaling regulates sensory cell migration in zebrafish. Dev Biol. 2004, 269: 123-36. 10.1016/j.ydbio.2004.01.020.View ArticlePubMedGoogle Scholar
- Yasuoka A, Hirose Y, Yoda H, Aihara Y, Suwa H, Niwa K, Sasado T, Morinaga C, Deguchi T, Henrich T, Iwanami N, Kunimatsu S, Abe K, Kondoh H, Furutani-Seiki M: Mutations affecting the formation of posterior lateral line system in Medaka, Oryzias latipes . Mech Dev. 2004, 121: 729-738. 10.1016/j.mod.2004.03.032.View ArticlePubMedGoogle Scholar
- Balabanian K, Lagane B, Infantino S, Chow KY, Harriague J, Moepps B, Arenzana-Seisdedos F, Thelen M, Bachelerie F: The chemokine SDF-1/CXCL12 binds to and signals through the orphan receptor CXCR7 in T lymphocytes. J Biol Chem. 2005, 280: 35760-35766. 10.1074/jbc.M508234200.View ArticlePubMedGoogle Scholar
- Burns JM, Summers BC, Wang Y, Melikian A, Berahovich R, Miao Z, Penfold MET, Sunshine MJ, Littman DR, Kuo CJ, Wei K, McMaster BE, Wright K, Howard MC, Schall TJ: A novel chemokine receptor for SDF-1 and I-TAC involved in cell survival, cell adhesion, and tumor development. J Exp Med. 2006, 203: 2201-2213. 10.1084/jem.20052144.PubMed CentralView ArticlePubMedGoogle Scholar
- Thisse B, Pflumio S, Fürthauer M, Loppin B, Heyer V, Degrave A, Woehl R, Lux A, Steffan T, Charbonnier XQ, Thisse C: Expression of the zebrafish genome during embryogenesis (NIH RO1 RR15402). ZFIN Direct Data Submission. 2001Google Scholar
- Zhou N, Luo Z, Luo J, Liu D, Hall JW, Pomerantz RJ, Huang Z: Structural and functional characterization of human CXCR4 as a chemokine receptor and HIV-1 co-receptor by mutagenesis and molecular modeling studies. J Biol Chem. 2001, 276: 42826-42833. 10.1074/jbc.M106582200.View ArticlePubMedGoogle Scholar
- Doranz B, Orsini M, Turner J, Hoffman T, Berson J, Hoxie J, Peiper S, Brass L, Doms R: Identification of CXCR4 domains that support coreceptor and chemokine receptor functions. J Virol. 1999, 73: 2752-2761.PubMed CentralPubMedGoogle Scholar
- Zhou N, Luo Z, Luo J, Fan X, Cayabyab M, Hiraoka M, Liu D, Jan X, Pesavento J, Dong C, Wang Y, An J, Kaji H, Sodroski JG, Huang Z: Exploring the stereochemistry of CXCR4-peptide recognition and inhibiting HIV-1 entry with D-peptides derived from chemokines. J Biol Chem. 2002, 277: 17476-17485. 10.1074/jbc.M202063200.View ArticlePubMedGoogle Scholar
- Kimmel CB, Ballard WH, Kimmel SR, Ullmann B, Schilling TS: Stages of embryonic development of the zebrafish. Dev Dyn. 1995, 203: 253-310.View ArticlePubMedGoogle Scholar
- Ledent V: Postembryonic development of the posterior lateral line in zebrafish. Development. 2002, 129: 597-604.PubMedGoogle Scholar
- Sapède D, Gompel N, Dambly-Chaudière C, Ghysen A: Cell migration in the postembryonic development of the fish lateral line. Development. 2002, 129: 605-615.PubMedGoogle Scholar
- Lopez-Schier H, Starr CJ, Kappler JA, Kollmar R, Hudspeth AJ: Directional cell migration establishes the axes of planar polarity in the posterior lateral-line organ of the zebrafish. Dev Cell. 2004, 7: 401-412. 10.1016/j.devcel.2004.07.018.View ArticlePubMedGoogle Scholar
- Villablanca E, Renucci A, Sapède D, Lec V, Soubiran F, Sandoval P, Dambly-Chaudière C, Ghysen A, Allende M: Control of cell migration in the zebrafish lateral line: implication of the gene "tumour-associated calcium signal transducer", tacstd . Dev Dyn. 2006, 235: 1578-1588. 10.1002/dvdy.20743.View ArticlePubMedGoogle Scholar
- Doitsidou M, Reichman-Fried M, Stebler J, Koprunner M, Dorries J, Meyer D, Esguerra CV, Leung T, Raz E: Guidance of primordial germ cell migration by the chemokine SDF-1. Cell. 2002, 111: 647-659. 10.1016/S0092-8674(02)01135-2.View ArticlePubMedGoogle Scholar
- Knaut H, Werz C, Geisler R, Nusslein-Volhard C, Tubingen 2000 Screen Consortium: A zebrafish homologue of the chemokine receptor Cxcr4 is a germ-cell guidance receptor. Nature. 2003, 421: 279-282. 10.1038/nature01338.View ArticlePubMedGoogle Scholar
- Molyneaux KA, Zinszner H, Kunwar PS, Schaible K, Stebler J, Sunshine MJ, O'Brien W, Raz E, Littman D, Wylie C, Lehmann R: The chemokine SDF1/CXCL12 and its receptor CXCR4 regulate mouse germ cell migration and survival. Development. 2003, 130: 4279-4286. 10.1242/dev.00640.View ArticlePubMedGoogle Scholar
- Sapède D, Rossel M, Dambly-Chaudière C, Ghysen A: Role of SDF1 chemokine in the development of lateral line efferent and facial motor neurons. Proc Nat Acad Sci USA. 2005, 102: 1714-1718. 10.1073/pnas.0406382102.PubMed CentralView ArticlePubMedGoogle Scholar
- Muller A, Homey B, Soto H, Ge N, Catron D, Buchanan ME, McClanahan T, Murphy E, Yuan W, Wagner SN, Barrera JL, Mohar A, Verastegui E, Zlotnik A: Involvement of chemokine receptors in breast cancer metastasis. Nature. 2001, 410: 50-56. 10.1038/35065016.View ArticlePubMedGoogle Scholar
- Gomperts M, Garcia-Castro M, Wylie C, Heasman J: Interactions between primordial germ cells play a role in their migration in mouse embryos. Development. 1994, 120: 135-141.PubMedGoogle Scholar
- Friedl P, Hegerfeldt Y, Tucsh M: Collective cell migration in morphogenesis and cancer. Int J Dev Biol. 2004, 48: 441-449. 10.1387/ijdb.041821pf.View ArticlePubMedGoogle Scholar
- Han Y, He T, Huang D, Pardo CA, Ransohoff RM: TNF-α mediates SDF-1α-induced NF-κB activation and cytotoxic effects in primary astrocytes. J Clin Invest. 2001, 108: 425-435. 10.1172/JCI200112629.PubMed CentralView ArticlePubMedGoogle Scholar
- Helbig G, Christopherson KW, Bhat-Nakshatri P, Kumar S, Kishimoto H, Miller KD, Broxmeyer HE, Nakshatri H: NF-κB promotes breast cancer cell migration and metastasis by inducing the expression of the chemokine receptor CXCR4. J Biol Chem. 2003, 278: 21631-21638. 10.1074/jbc.M300609200.View ArticlePubMedGoogle Scholar
- Kollmar R, Nakamura SK, Kappler JA, Hudspeth AJ: Expression and phylogeny of claudins in vertebrate primordia. Proc Natl Acad Sci USA. 2001, 98: 10196-10201. 10.1073/pnas.171325898.PubMed CentralView ArticlePubMedGoogle Scholar
- Westerfield M: The zebrafish book: a guide for the laboratory use of the zebrafish (Danio rerio). 1994, Eugene: University of Oregon Press, 2Google Scholar
- Hauptmann G, Gerster T: Two-color whole-mount in situ hybridization to vertebrate and Drosophila embryos. Trends Genet. 1994, 10: 10-Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.