CONDOR: a database resource of developmentally associated conserved non-coding elements
© Woolfe et al; licensee BioMed Central Ltd. 2007
Received: 16 June 2007
Accepted: 30 August 2007
Published: 30 August 2007
Comparative genomics is currently one of the most popular approaches to study the regulatory architecture of vertebrate genomes. Fish-mammal genomic comparisons have proved powerful in identifying conserved non-coding elements likely to be distal cis-regulatory modules such as enhancers, silencers or insulators that control the expression of genes involved in the regulation of early development. The scientific community is showing increasing interest in characterizing the function, evolution and language of these sequences. Despite this, there remains little in the way of user-friendly access to a large dataset of such elements in conjunction with the analysis and the visualization tools needed to study them.
Here we present CONDOR (COnserved Non-coDing Orthologous Regions) available at: http://condor.fugu.biology.qmul.ac.uk. In an interactive and intuitive way the website displays data on > 6800 non-coding elements associated with over 120 early developmental genes and conserved across vertebrates. The database regularly incorporates results of ongoing in vivo zebrafish enhancer assays of the CNEs carried out in-house, which currently number ~100. Included and highlighted within this set are elements derived from duplication events both at the origin of vertebrates and more recently in the teleost lineage, thus providing valuable data for studying the divergence of regulatory roles between paralogs. CONDOR therefore provides a number of tools and facilities to allow scientists to progress in their own studies on the function and evolution of developmental cis-regulation.
By providing access to data with an approachable graphics interface, the CONDOR database presents a rich resource for further studies into the regulation and evolution of genes involved in early development.
In recent years, although great advances have been made in our ability to annotate gene sequences, the annotation of sequence elements that control complex gene expression is lagging far behind. With exons accounting for only a tiny proportion of the genome (< 1.5%) there remains a vast amount of unannotated sequence, containing an unknown number of functional elements. In particular, the annotation of the complex network of cis-regulatory modules (CRMs), such as enhancers and silencers, that coordinate the precise spatial and temporal expression of genes during development presents one of the biggest challenges of the post-genomic era. This is predominantly because we know so little about their language, mode of action and origin, and traditional ways of identifying them are difficult and laborious. The burgeoning field of evolutionary developmental biology (termed evo-devo) is increasingly shifting its emphasis towards the importance of gene regulatory networks in the evolution of developmental pathways . Within evo-devo there is therefore growing interest in the identification and characterization of the CRMs that control these networks. With the completion of an increasing number of vertebrate genomes the correlation between sequence conservation and biological function provides a powerful resource for the discovery of functional elements. Indeed a number of recent large-scale comparative studies have resulted in the identification of thousands of evolutionary constrained elements in the human genome (e.g. [2–5]). These conserved non-coding sequences represent a diverse set of functional elements, a proportion of which are likely to act as CRMs.
The confidence with which putative functional non-coding elements can be identified depends on the evolutionary distance between the species selected, and a balance must be found between eliminating background noise and retaining sufficient sequence similarity for detection . The inclusion of fish genomes in comparative analysis has proved a highly successful approach to filter and prioritize non-coding sequences most likely to be functional.
The large evolutionary distance between fish and mammals means that even the slowest evolving DNA has diverged sufficiently to significantly improve the signal to noise ratio in genomic alignments. In particular the genome of the pufferfish, Fugu rubripes, with its highly compact non-coding regions, has been commonly used as a model genome to increase the efficacy of detecting putative functional sequences. The similarities between mammals and Fugu are recognisable despite their large evolutionary divergence. Both vertebrates, they share not only the defining characteristic of a backbone, but also many basic anatomical and physiological similarities and patterns of development. The strong parallels in early developmental patterning are likely to be under control of a common core set of genes and regulatory elements. Significantly, a strong spatial association between highly conserved non-coding elements (CNEs) and genes involved in transcriptional regulation and/or development (termed trans-dev genes) has been described in many studies [3, 4, 7–9]. Functional testing has shown that conserved non-coding elements identified using very strict conservation criteria in mammals (e.g. ultraconserved elements ) or using evolutionary distant species [4, 10] are considerably enriched for enhancer sequences. In both mouse  and zebrafish , a large proportion of the elements tested in-vivo function reproducibly to up-regulate tissue-specific expression of reporter genes during development.
With an increasing supply of functional data, we have created CONDOR (Conserved Non-coDing Orthologous Regions), a database resource for the study of evolutionary conserved cis-regulation in early developmental genes. Here, such elements are rigorously defined by optimal alignment strategies for sequences as divergent as fish and tetrapods, ensuring that sequences are derived from orthologous regions. In addition, we cater for the growing number of researchers in evo-devo interested in sets of experimentally verified developmental enhancers. Thus we couple both bioinformatics and functional data in a visual and searchable form.
Construction and content
The relational database structure can be divided conceptually into two main parts, representing the two main sources of data stored within it. The first relates to the computationally derived dataset from Fugu-mammal multiple alignments, in particular the CNEs identified from them. The second stores functional (lab-based) data from in vivo enhancer assays carried out in-house. While these two datasets are integrated in the database their derivation will be outlined separately.
Numbers of CNEs in each region in CONDOR vary considerably, ranging from two in the PAX8 region to 342 in the IRX3/5/6-SALL1 region, possibly reflecting differences in regulatory complexity and the extent to which regulation has been conserved between fish and mammals as well as the possible presence of overlapping regulatory domains. CNEs in CONDOR range in length from 21–864 bp (mean = 117 bp) and cover some 750 Kb of human sequence, or around 0.05% of the non-repetitive genome. Therefore, although this set represents a tiny fraction of the ~5% of the human genome thought to be under selective constraint  it is proven to be of the highest regulatory potential in development [4, 23, 11].
It is important for future analyses that published sets of tested CRMs identified through species comparisons can in some way be traced back to a sequence repository and thus properly defined, similar to the definition of sequences in protein or transcript databases. Commonly in currently published work, functionally characterized conserved non-coding sequences are referred to by some arbitrary name and by their location in the genome or the distance from the start of a gene. This often changes with the release of updated genome assemblies and makes it difficult for other researchers to track the sequence back from the literature. In response, CONDOR is the first database to use specific identifiers for conserved non-coding elements in each genome, independent of their current location in a genome assembly. Unique identifiers are used both to refer to a CNE orthologous unit (akin to a cluster of orthologous genes), e.g. CRCNE00000054, which is useful in defining a CNE in any vertebrate genome to which it is conserved, as well as individual sequences within the CNE, e.g. CRCNEAC00000172 (in human), from sequences used to define the element in the original multiple alignment (i.e. human, mouse, rat, dog and Fugu). Specific identifiers are only assigned to sequences in these species (as opposed to any of the other vertebrate genome in which a CNE is present), as they are only sequences that can be strictly guaranteed to derive from orthologous regions.
In vivoenhancer assay dataset
The CONDOR database also serves as a repository of CNEs experimentally tested in vivo for enhancer activity, with around 100 elements currently tested. Details of this in vivo assay using the zebrafish model Danio rerio have been published previously. Briefly, as candidate enhancer elements, CNEs are PCR amplified using primers designed as close to the conserved sequence as possible. This guarantees that as far as possible any activity from the assay derives directly from the conserved sequence and not from undetected regulatory signals in non-conserved flanking sequence. Other enhancer assays (e.g.  and ) often include large regions of sequence that flank the conserved element, making it increasingly difficult to narrow down the sequences responsible for enhancer activity. The PCR product is co-injected together with a GFP reporter construct under the control of a human β-globin minimal promoter into zebrafish embryos before the eight cell stage. Embryos are generally screened for GFP expression 24–30 hours post fertilization (hpf) and again at 48–54 hpf. It is not thought that the injected DNA is integrated into the genome leading to mosaic expression. Therefore for each assay, we generate GFP expression data from a minimum of 25 embryos in order to obtain an overall expression profile. Using graphics software, this profile is depicted schematically as color-coded cells drawn onto an overlay of a camera-lucida drawing of either a 24–30 hpf or 48–54 hpf embryo. The color-coding relates to nine broad expression domains (e.g. central nervous system, sensory organs etc) that can be visualized as separate schematics. These are further subdivided into one or more localized expression domains (e.g. forebrain, midbrain and hindbrain) and the proportion of embryos with expression in each sub-domain is shown as a bar chart.
The transparency and rapid development of zebrafish renders it a popular model organism for developmental biology. Unlike the mouse model, large numbers of embryos can be screened and GFP expression can be readily monitored in a live embryo. Also mouse embryos are opaque, and so without dissection, low levels of expression may remain undetected. Since complementary results can be obtained when assaying human-fish conserved elements in either model system , data from our in vivo assay represents a valuable foundation for prioritizing more focused studies in a mammalian system. As with any assay system there are a number of caveats associated with interpreting results. Elements are tested out of their genomic context and may not produce the same patterns of expression as they would in the genome, possibly due to structural or steric influences or interactions with other elements not present in the assay. In addition, CNEs that test 'negative' in our assay may act as enhancers in time points beyond the assay, or in rare cell types. Alternatively, they may be acting as another type of functional element such silencers, structural regulators or ncRNAs. Users should therefore take these caveats into consideration.
The number of tested elements in the database will continue to grow in the future as in-house gene-centric studies continue and new techniques are employed and developed (such as a Tol2 integration  and recombineering), although no systematic assaying of all elements in the database is currently planned. The number of labs using zebrafish embryos to assay developmentally associated CNEs for enhancer activity is increasing rapidly. We therefore invite scientists using this assay to contribute their annotations from such studies (following publication) to CONDOR to make the database a community resource and allow those results to become more widely available and distributed.
Utility and discussion
Both comparative genomic data and in vivo results are publicly available as a web-based resource. The website is designed to make the interrogation and visualisation of this data as user friendly and intuitive as possible, catering for both lab-based investigators interested in biological function and bioinformatics researchers interested in sequences datasets of high regulatory potential. The web site therefore provides a number of approaches by which the data can be accessed, visualised and downloaded.
Interrogation and visualization of CNEs by genomic region
The central search page allows CNE-containing gene regions to be located from a pull-down list or searched more generally through a text search by gene name, protein accession number, GO term etc for any genes contained within these regions. Once located, CNEs within a gene region can be visualized via an interactive graphical browser (termed 'CONDOR View') or in a tabular form (termed 'Text View'). Each gene region analysed extends as far as the shared synteny and gene order between fish and mammals, allowing for long-range cis-regulatory elements that may lie within or beyond neighbouring genes. These regions are represented in CONDOR View so that users can graphically view the positions of CNEs along a chosen baseline genome in relation to other vertebrate CNEs. The conservation or absence of CNEs in other genomes used in the initial multiple alignment are also indicated through separate tracks in the browser. An example of 'CONDOR View' for CNEs in the SOX21 region in Fugu can be seen in Figure 3. By mousing over a CNE, simple information such as position, length, conservation etc. is displayed and an overview of enhancer activity is also shown for elements that have been tested (Figure 3). Users can zoom in and out of the browser to view specific regions in more detail (particularly useful in very large regions spanning several megabases) as well as select only CNEs that have been tested for enhancer activity. Directly clicking on the CNE in the browser opens a separate webpage with detailed sequence, evolutionary and in-vivo assay data (if available).
The database is also available as a permanent DAS source within the most current human, mouse and rat assemblies at the Ensembl Genome Browser  so that any overlap between the CNEs and an increasing number of in silico and experimental annotations can be readily viewed. A similar track in the UCSC Genome Browser is also planned in the future.
Searching CNEs by enhancer activity
The vast majority of CNEs exhibit no sequence identity to any other sequence in the genome [4, 28, 29]. However, their seemingly unique nature stands in contrast to their apparent ability to drive expression to similar anatomical locations [4, 11, 23]. Therefore, while CNEs show little intra-genomic conservation at the sequence level, they are likely to share small common motifs representing protein-binding sites not readily detectable by sequence conservation algorithms. Indeed, a recent study of a broader set of conserved elements in mammals identified more than 200 overrepresented motifs, raising the possibility that specific motifs may be associated with specific classes of functional elements . Clustering CNEs by their spatial and temporal specific enhancer activity to look for similar common motifs may therefore allow us to begin to discern the language of developmental enhancers. CONDOR provides a search facility that allows a user to search for tested CNEs driving expression in any of 19 anatomically distinct tissues and two developmental time points in zebrafish embryos. Figure 5 gives an example of search results for CNEs driving strong expression in the eye. A multi-FASTA file of all the resultant sequences is also returned to allow for further downstream analyses such as searching for common motifs or known transcription factor binding sites.
CONDOR will enable greater accessibility to ongoing functional data of cis-regulatory elements complementing and adding to the growing list of enhancers tested in the mouse model  with the added advantage of more developmental time points in zebrafish. As more annotated enhancers become available (in particular those confirmed by more than one assay system) statistical approaches aimed at deriving a regulatory language from this training set should become attainable.
Studying duplicated elements and regulatory subfunctionalization
Whilst the vast majority of CNEs conserved across vertebrates are unique in the human genome [4, 29] an interesting subset are part of multi-member families that share significant sequence similarity and derive from ancient whole-genome duplications at the origin of vertebrates . These dCNEs (duplicated CNEs) are located in the vicinity of trans-dev paralogs and offer an important resource for studying cis-regulatory element evolution after duplication and how this influences paralogous gene function. It is worth noting that since CNEs can act over a long range, identifying their target gene can become complex when studying a region that contains more than one developmental gene. In the case of dCNEs, the retention of both element and gene after duplication, strongly implies association . CONDOR highlights those CNEs that are duplicated (see Figure 4 as an example) and provides a tool to create alignments and locate genomic positions of all other members in any of the vertebrate genomes searched.
In addition, the CONDOR database is the first resource to make available data on CNEs identified from a number of teleost-specific duplicated regions within Fugu from alignments where both Fugu gene regions are compared independently to the same (ancestral) region in mammals. Recent comparative analysis of CNEs in these regions reveals assymetrical evolutionary rates and a pattern of retention and loss of CNEs between duplicated genes indicative of regulatory subfunctionalization . To facilitate their study, CONDOR supplies an extra facility that allows the CNEs in these duplicated regions to be compared side-by-side highlighting those CNEs that have partitioned to a specific gene copy as well as those conserved to some degree in both copies.
Additional tools and features
In addition to providing data on the locations and sequence information of each CNE, CONDOR also provides a number of other features to help study the function and evolution of these sequences:
• To facilitate the amplification of CNEs from a genome of choice for in vivo or in vitro analysis, a number of automatically generated PCR primer predictions using the EPrimer3 program  are provided for all CNEs. Alternatively, flanking sequence around the CNE of a user-defined size can be downloaded to allow manual primer design.
• Sensitive BLAST alignments of any CNE against a variety of nucleotide sequence databases can be displayed dynamically (where the user can choose the e-value threshold of results displayed). Database searches include a variety of vertebrate genomes (see Figure 3) as well as transcribed sequence databases such as EMBL EST and Unigene and ncRNA databases that are updated on a regular basis. This extensive resource has a number of uses, predominant of which is identifying those genomes in which the CNE has been duplicated in lineage (such as those at the origin of vertebrates  or within the teleosts ) or species-specific duplication events.
• To aid evolutionary analyses of CNEs, and utilising the BLAST results, a user can choose any combination of vertebrate genome from which to produce a multiple alignment of the CNE (from a choice of two aligners) allowing lineage or species specific mutational events to be detected. The output produces the multiple alignment in a number of formats (such as FASTA, MSF etc) that can be directly viewed in the browser or downloaded for further downstream analysis.
• CNEs from each of the main genomes can be downloaded in bulk as a set of sequences in FASTA format or as a tab-delimited file with substantial sequence and positional data. Bulk downloads can also be filtered by genome, gene region or CNE length and conservation.
The database is run on a Solaris platform and housed using the MySQL (v1.4.0) database management system at the School of Biological and Chemical Sciences, QMUL, London. The web interface is run on an Apache web server that serves a set of CGI scripts and static web pages. The "CONDOR-View" graphical browser is created using the Bioperl Bio::Graphics module . CNEs are served to the Ensembl Genome browser using the ProServer DAS server (v2) .
Availability and requirements
The CONDOR database is publicly available without payment or registration at http://condor.fugu.biology.qmul.ac.uk. A full tutorial can be found on the website. Please cite this paper when publishing data using computational data from CONDOR. Use of functional data in publications is only permitted with prior agreement from the corresponding author. Positions of CNEs within vertebrate genome assemblies are constantly updated as new versions are released and in vivo zebrafish enhancer annotations are added to the database automatically once results for each experiment are collated.
This paper is dedicated to the memory of Sarah Smith, our colleague and friend. We would like to thank Mike Brudno for hands-on help on getting the most from MLAGAN/SLAGAN and Jonathan Crabtree and Lincoln Stein for help on using the BioPerl Bio::Graphics module. We would also like to thank Peter Tribble for setting up and maintaining the server upon which the CONDOR database and website runs. This work was funded by the UK Medical Research Council. All authors have read and approved the manuscript.
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