Lectin binding profiles of SSEA-4 enriched, pluripotent human embryonic stem cell surfaces
© Venable et al; licensee BioMed Central Ltd. 2005
Received: 25 March 2005
Accepted: 21 July 2005
Published: 21 July 2005
Pluripotent human embryonic stem cells (hESCs) have the potential to form every cell type in the body. These cells must be appropriately characterized prior to differentiation studies or when defining characteristics of the pluripotent state. Some developmentally regulated cell surface antigens identified by monoclonal antibodies in a variety of species and stem cell types have proven to be side chains of membrane glycolipids and glycoproteins. Therefore, to examine hESC surfaces for other potential pluripotent markers, we used a panel of 14 lectins, which were chosen based on their specificity for a variety of carbohydrates and carbohydrate linkages, along with stage specific embryonic antigen-4 (SSEA-4), to determine binding quantitation by flow cytometry and binding localization in adherent colonies by immunocytochemistry.
Enriching cells for SSEA-4 expression increased the percentage of SSEA-4 positive cells to 98–99%. Using enriched high SSEA-4-expressing hESCs, we then analyzed the binding percentages of selected lectins and found a large variation in binding percentages ranging from 4% to 99% binding. Lycopersicon (tomato)esculetum lectin (TL), Ricinus communis agglutinin (RCA), and Concanavalin A (Con A) bound to SSEA-4 positive regions of hESCs and with similar binding percentages as SSEA-4. In contrast, we found Dolichos biflorus agglutinin (DBA) and Lotus tetragonolobus lectin (LTL) did not bind to hESCs while Phaseolus vulgaris leuco-agglutinin (PHA-L), Vicia villosa agglutinin (VVA), Ulex europaeus agglutinin (UEA), Phaseolus vulgaris erythro-agglutinin (PHA-E), and Maackia amurensis agglutinin (MAA) bound partially to hESCs. These binding percentages correlated well with immunocytochemistry results.
Our results provide information about types of carbohydrates and carbohydrate linkages found on pluripotent hESC surfaces. We propose that TL, RCA and Con A may be used as markers that are associated with the pluripotent state of hESCs because binding percentages and binding localization of these lectins are similar to those of SSEA-4. Non-binding lectins, DBA and LTL, may identify differentiated cell types; however, we did not find these lectins to bind to pluripotent SSEA-4 positive hESCs. This work represents a fundamental base to systematically classify pluripotent hESCs, and in future studies these lectins may be used to distinguish differentiated hESC types based on glycan presentation that accompanies differentiation.
Ever since the isolation of human embryonic stem cells (hESCs) in 1998 , the implications for their use in a number of disease therapies have been highly regarded. Additionally, these cells also find value as a model to study basic human development. However, in all aspects of ESC research, hESCs must first be appropriately defined or characterized. One way to characterize hESCs is to utilize the large number of glycoproteins and carbohydrates existing on the cell surface as a way to delineate pluripotent or differentiated cell types.
The most common hESC surface pluripotency markers are the stage specific embryonic antigens -3 and -4 (SSEA-3, -4) and tumor rejection antigens-1-60 and -1-81 (TRA-1-60, -1-81). SSEA-3 and -4 are globoseries cell surface glycoproteins that were first used to delineate embryological changes in the developing mouse embryo [2, 3]. Both of these antigens were found to recognize sequential regions of a mouse ganglioside epitope, with SSEA-4 (MC813-70 antibody) recognizing the terminal portion of the sequence and SSEA-3 (MC613) recognizing the internal region of he sequence. Thus, two antibodies were used to define this unique embryonic antigen. In mouse embryonic stem cells (mESCs), SSEA-3 and -4 are expressed on the 2–8 cell and morula stages of preimplantation embryos and are also found on unfertilized oocytes; however, there is a loss of expression in the inner cell mass (ICM) of mESCs [2, 3]. Yet in hESCs, there is no expression of SSEA-3 or -4 at the 2–8 cell or morula stage; however, these are expressed on the ICM of human blastocysts and on isolated hESCs . It has been well documented that these cell surface carbohydrates change both with development and differentiation in vitro [5, 6], but there may be other undiscovered developmentally regulated cell surface carbohydrates.
The hematopoetic field has numerous cell surface antigens which have been identified that define various bone marrow and blood stem cells, such as CD4, CD8, CD34, CD38, CD44, CD45, c-kit, Mac-1, Muc -18, Lin, Sca-1, and Thy-1 [7–11] and there has been some progress in identifying other hESC surface markers that can be used both for defining the pluripotent state and for cell sorting and cell identification. Cell surface markers such as CD9 and CD24 are presented on pluripotent hESC surfaces, while gene expression analysis indicates such genetic markers as REX1 , Cripto/TDGF1 , OCT-4 , DNMT3B , LIN28 , Nanog , and others that can denote pluripotency in hESCs . Since carbohydrate antigens on hESC surfaces could provide further potential markers defining the pluripotent state, we targeted glycosylation patterns on the cell surface of SSEA-4 enriched hESCs using lectins to bind to these glycans. Lectins, carbohydrate binding proteins that recognize diverse sugar structures, have been extensively used to identify and characterize cell surface glycosylation patterns. For example, lectins have been used to investigate metastatic processes in many types of cancer [15–21] and to identify cell types based on presentation of specific cell surface carbohydrates [22–27]. Carbohydrate analysis using lectins has also led to the delineation of embryologic developmental stages in some species. For example, many developmentally regulated glycans identified as lectin receptors on mESCs are displayed on cell surfaces at the preimplantation and implantation stages of development. These include Concanavalin A (Con A) defined by the hapten methylmannoside , Peanut agglutinin (PNA) from Arachis hypogaea defined by the hapten galactose , Wheat Germ Agglutinin (WGA) from Triticum vulgaris defined by hapten N-acetylglucosamine , Dolichos biflorus agglutinin (DBA) defined by the hapten N-acetylgalactosamine , and Ricinus communis agglutinin (RCA) defined by the hapten galactose or lactose . These results suggest that glycans may be involved in cell-cell interactions serving a specific developmental function and also indicate that they can be used as markers to define these stages of mouse embryogenesis.
We found that a variety of lectins binding unique carbohydrate moieties had distinct binding patterns. Our findings demonstrate that there are many surface carbohydrate antigens that could be exploited to further characterize the pluripotent state of hESCs, and these lectins may provide a source of unique markers with which to characterize subpopulations that exist in colonies of adherent hESCs.
Carbohydrate analysis using flow cytometry
Two lectins, DBA and LTL, did not bind to hESCs. Analysis of DBA and LTL histograms showed no peak shifts using these lectins (Figure 4C), and analysis of flow cytometry plots also did not reveal any peak shifts (supplementary data).
Some lectins were found to partially bind to hESC colonies. These lectins revealed two distinct subpopulations of cells that included a SSEA4+/lectin- population and a SSEA4+/lectin+ population. Figure 4D shows a representative histogram plot of MAA which shows a large population of cells that overlap the unstained control cells; however, the arrow indicates another smaller population of cells that show a peak shift representing positive MAA binding. Figure 4E shows the comparison of unstained control cells to the shifted plot of MAA (Figure 4F) in which there are two distinct populations of cells present: a SSEA-4+/lectin- population containing the majority of cells, and a smaller but still distinct SSEA-4+/lectin+ population indicated by the arrow. Lectins that bound in this way included PHA-E, VVA, UEA, PHA-L, and MAA, and these exhibited a large binding range varying from 58% to 13% binding of enriched SSEA-4 positive cells (Figure 3).
Carbohydrate analysis using immunocytochemistry
We also observed interesting patterns of carbohydrate expression for the lectins that showed two subpopulations of cells when analyzed using flow cytomtery. VVA bound to distinct regions of a SSEA-4 positive colony (Figure 5D–F), in contrast to the more uniform binding described above. In Figure 5D the arrow shows a region where there is no VVA binding, but this region is SSEA-4 positive as shown in Figure 5E. The DAPI nuclear stain of this same colony is shown in Figure 5F. Figures 5G & H show PHA-E binding in different colonies. In Figure 5G there are two adjacent colonies with one colony showing strong binding of SSEA-4 antibody (green) and little PHA-E binding (red). The adjacent colony shows binding of PHA-E but a lack of SSEA-4 antibody binding. In Figure 5H there is one colony that shows a streak of stacked cells as determined by the high expression of DAPI staining in the middle of the colony (depicted by arrow). These cells are beginning to lose SSEA-4 antibody binding (green) but have strong PHA-E binding (red). The rest of the colony adjacent to this streak of cells is uniformly positive for SSEA-4 but lacks PHA-E binding.
Using immunocytochemistry we also demonstrated that DBA and LTL did not bind to hESC colonies. Figure 5I shows a hESC colony which is SSEA-4 positive (green) and is stained with DAPI nuclear stain (blue) but shows only debris of the DBA lectin (red) with no regions of DBA binding.
Validation of lectin binding
Comparison of the specificity for monosaccharides and oligosaccharides of a panel of 14 biotinylated lectins used in immunocytochemistry and flow cytometry.
Concanavalin A (Con A)
Man or Glc
200 mM α-methylmannoside or α-methyl glucoside
Phaseolus vulgaris erythro-agglutinin (PHA-E)
Phaseolus vulgaris leuco-agglutinin (PHA-L)
Sambucus nigra agglutinin (SNA)
500 mM lactose in acetic acid
Arachis hypogea peanut (PNA)
200 mM galactose
Vicia villosa agglutinin (VVA)
200 mM N-acetylgalactosamine
Maackia amurensis (MAA)
200 mM lactose
Ricinus communis agglutinin (RCA)
200 mM galactose or lactose
Wisteria floribunda agglutinin (WFA)
200 mM N-acetylgalactosamine
Ulex europaeus agglutinin (UEA)
50–100 mM L-fucose
Lotus tetragonolobus lectin (LTL)
50–100 mM L-fucose
Dolichos biflorus agglutinin (DBA)
200 mM N-acetylgalactosamine
Hippeastrum hybrid lectin (HHL)
100 mM mannose
Lycopersicon esculetum tomato (TL)
This study is the first of which we are aware to use lectins to probe the surface of hESCs in order to analyze carbohydrate presentation. To quantitate lectin binding percentages on pluripotent hESC surfaces using flow cytometry, we first enriched our colonies for SSEA-4 expression. Using enriched pluripotent hESCs, we found a large binding percentage range for this chosen panel of 14 lectins. Some lectins bound throughout the colonies of pluripotent hESCs, and the carbohydrate moieties represented by this group include alpha-linked mannose (Con A), galactosyl(β1–3) N-acetylgalactosamine (PNA), terminal galactose and N-acetylgalactosamine (RCA), GalNAc β4-Gal (WFA), N-acetylglucosamine (TL) and sialic acid alpha 2,6 GalNAc (SNA) and alpha-linked mannose residues only (HHL). These results indicate that there is high expression of these carbohydrates with these particular linkages on pluripotent hESC surfaces. As ConA can bind both mannose and glucose moieties, we see greater lectin binding of ConA as opposed to HHL, which specifically binds only mannose residues. These lectins may be additional markers to denote pluripotent hESCs. Similarly, WFA, PNA and SNA were found to bind to over 60% of hESCs, suggesting that these lectins could be indicative of a pluripotent phenotype as well; however, they do not have binding percentages that correlate with SSEA-4 expression.
We also found that some lectins showed interesting partial binding patterns. Two distinct populations of cells were observed as determined by flow cytometry plot analysis and confirmed by immunocytochemistry using these lectins and SSEA-4 antibody. These hESC populations were identified as either SSEA-4+/lectin+ or SSEA-4+/lectin- regions. Lectins showing this binding pattern included PHA-L, VVA (see Figure 5D–F), UEA, PHA-E (Figure 5G–H), and MAA. Table 1 shows their carbohydrate binding specificities.
As shown by immunocytochemistry of the representative lectin, PHA-E, it appeared that some areas that were beginning to lose expression of SSEA-4, show binding of the lectin (Figure 5G–H). A decrease of SSEA-4 expression can occur even under the most stringent culturing conditions and in this research, is important because it underscores the need for enriching for desired populations of cells prior to experimentation and also supports the hypothesis that cell-cell interactions are important in carbohydrate presentation. As clusters of cells lose SSEA-4 expression, and therefore differentiate, new carbohydrates are presented on the cell surface. Furthermore, these differentiated cells could be indicative of progenitor subpopulations existing in the colonies. For example, another lectin in our study that was found to bind partially to colonies is UEA, which binds fucose moieties and has been determined to be a marker for endothelial cells . The role these partially presented carbohydrates play was not investigated in this study, but others have suggested that certain carbohydrates may serve a developmental function. For example, Panin and colleagues have identified O-fucose on epidermal growth factor-like repeats of Notch, and elongation of O-fucose has been implicated in the modulation of Notch signaling by Fringe [36, 37]. Notch receptors and associated proteins are important in a number of signaling pathways that direct cell fate decisions, proliferation and apoptosis.
We also observed that two lectins, DBA and LTL, did not bind hESCs. DBA binds primarily to α-linked N-acetylgalactosamine and LTL binds fucose moieties that are α-linked to GlcNAc. These non-binding lectins suggest that these particular carbohydrates and their associated linkages are either not present on pluripotent hESC surfaces or if they are present, they have been modified and therefore are not susceptible to lectin binding. Interestingly, however, PNA and WFA, which bind to a high percentage of hESCs, bind to β-linked N-acetylgalactosamine. Thus, it appears that the linkage of this carbohydrate is important. Similarly, UEA binds to about 20% of our cells and recognizes fucose moities like LTL. However, the linkages that these lectins recognize are different and cause different binding percent outcomes, further suggesting that the linkage of these carbohydrates are important.
Developmentally regulated cell surface antigens have been identified by monoclonal antibodies in a variety of species and stem cell types. Many of these antigens have proven to be side chains of membrane glycolipids and glycoproteins. For example, the SB10 antigen found on human mesenchymal stem cells was shown to be the activated leukocyte-cell adhesion molecule (ALCAM) that possesses N-linked oligosaccharide side chains . Muramatsu and colleagues discovered a protein called embigin that has a developmentally regulated carbohydrate chain that is lost upon mouse embryogenesis . Furthermore, other researchers showed that the monoclonal antibody GCTM-2, which binds to human embryonic carcinoma cells and primate ES cells, recognizes an epitope on a keratan sulfate proteoglycan [40, 41]. These studies have shown not only that carbohydrates on cell surfaces change with embryological development but also that they can be used to identify different types of stem cells. In addition, there are a number of carbohydrate epitopes that have been found on the mammalian preimplantation embryo. Ofcourse, the tumor rejection antigens and stage specific antigens have been well characterized on human embryos , but there are many others such as the TEC antigens (TEC1- TEC4), LeX, LeY, CD46, CD55, and CD59. The TEC antigens have been shown to recognize either the carbohydrate moiety of embryoglycan or a developmentally regulated protein epitope on both mouse and bovine preimplantation embryos . LeX [Galbeta1-4(Fucalpha1-3)GlcNAc] is first detected on the blastomeres of the 8-cell stage of the mouse embryo and LeY [Fucalpha1-2Galbeta1-4(Fucalpha1-3)GlcNAc] is highly expressed on the surface of the mouse blastocyst [43, 44]. Also, studies of human preimplantation embryos show that the glycosyl phosphatidylinositol anchored proteins CD46, CD55 and CD59 are expressed on oocytes and plasma membrane . In this study, we analyzed glycans on the cell surface of hESCs using a panel of 14 lectins and SSEA-4 antibody and found that pluripotent hESC surfaces have a large variety of surface carbohydrates that can be targeted for marker sources.
This work represents a fundamental base to systematically classify pluripotent hESCs, and in future studies these lectins may be used to distinguish differentiated hESC types based on carbohydrate presentation that accompanies differentiation. It will be important to determine the potential of these lectin positive cell types and to identify the cell surface glycoproteins or glycolipids to which these lectins bind. Because there are many different enzymes that add or remove carbohydrate structures on cell surfaces, it will also be interesting to investigate the function of some of these glycosyltransferases in both pluripotent and differentiated hESCs.
Cell culture and passaging
NIH approved hESC lines BG01 and BG02 with normal karyotypes were obtained from BresaGen, Inc. . Cells were grown in DMEM/F12 supplemented with 15% FCS (HyClone), 5% knockout serum replacer, 1× non-essential amino acids, 20 mM L- Glutamine, 0.5 U/ml penicillin, 0.5 U/ml streptomycin, 4 ng/ml FGF-2 (Sigma), (all from Gibco Invitrogen unless otherwise labeled). Human ESCs were maintained on mitotically inactivated primary mouse embryonic fibroblasts (MEF) feeder layers for routine maintenance. Cells were grown in 100 cm tissue culture treated dishes (Falcon). Cells were passaged every 3 days using either a pretreatment with 10 mg/ml collagenase for 2 minutes followed by 0.05% trypsin for 1 minute or manual dissection with a fire pulled Pasteur pipette . Immunocytochemistry was performed on routinely maintained adherent hESC colonies, and flow cytometry was performed using routinely maintained hESC colonies that were first enriched for SSEA-4.
Enrichment of SSEA-4 positive cells
Cultures of hESCs were grown in 100 cm dishes and trypsin passaged into single cell suspensions as described above. Cells were incubated on ice for 15 minutes in 1:10 dilution of SSEA-4 (MC 813-70, Developmental Studies Hybridoma Bank [DSHA]; Iowa City; in stain buffer (SB). SB consisted of 0.5 ml 0.5 U/ml penicillin, 0.5 U/ml streptomycin, 0.5 ml of 100 mM EDTA, 46.5 ml phosphate buffered saline (PBS) and 2.5 ml fetal bovine serum (FBS). After incubation 10 ml SB was added and cells were resuspended and centrifuged at 3000 × g for 5 minutes. Supernatant was removed, leaving the cell pellet intact, and cells were washed again in SB and resuspended. After centrifugation, a 1:4 dilution of secondary anti-mouse IgG in stain buffer was added to the cell pellet, and the resuspended pellet was incubated on ice for 25 minutes. After incubation 10 ml SB was added to wash the cell pellet, followed by centrifugation (3000 × g) for 5 minutes. This process was repeated two more times in 5 ml SB. Cells were finally resuspended in 500 μl of SB before being applied to a pre-washed magnetic bead column. The flow through from the column was collected and saved for counting, and the retained eluate was collected separately. Both flow through and eluate were brought up to 5 ml in SB after collection and counted . Cells were then subjected to flow cytometry. Flow cytometry results presented here are from hESCs that were used immediately after the enrichment procedure.
Cell surface antigen and carbohydrate expression of SSEA-4+ enriched hESCs was assessed by indirect immunofluorescence detected by flow cytometry to provide a quantitative binding percentage of both SSEA-4 and lectin. Human ESCs were harvested into single cell suspensions using trypsinization as described above. Cells were enriched for SSEA-4 as described above. Then, cells were fixed in 2% paraformaldehyde in 1× PBS. After blocking in 1% bovine serum albumin (BSA) for 30 minutes, cells were placed in sterile conical tubes in aliquots of 500,000 cells each and double stained with one of the 14 lectins at 5 μg/ml and SSEA-4 in a 1:100 dilution. Cells were washed 3 times with PBS and then stained with secondary antibodies that included streptavidin -allophycocyanin (1:250, BD Biosciences; Franklin Lakes, NJ;) for recognition of biotinylated lectins and antigoat Mouse IgG conjugated Alexa 488; (1:2000) for recognition of SSEA-4. These secondary antibodies were chosen so that there would not be overlap in the emission/excitation wavelengths and so that double staining could be performed. Unstained, enriched hESCs and enriched hESCs stained with secondary antibodies alone were used as controls.
Table 1 shows the chosen tested lectins, their commonly abbreviated name, and the specificity of these lectins for their respective monosaccharides to act as a quick reference guide and the concentration of hapten, or competitive sugar, that was used to verify specificity of each lectin for its carbohydrate. Specificity of each lectin has previously been described in detail by Cummings and colleagues . Cytometry was performed using a Beckman Coulter Cytomics FC 500 Flow Cytometer. Data analysis was performed using the RXP Analysis Software by Beckman Coulter and Windows Multi Document Interface for Flow Cytometry (WinMDI 2.8). Three independent assays were carried out using both BG01 and BG02 hESC lines.
Immunocytochemistry was used to analyze the localization of cell surface carbohydrate expression and SSEA-4 on routinely maintained adherent cultures of hESCs. Human ESCs were harvested from 100 cm2 dishes by either trypsin/collagenase dissociation or manual dissociation and fixed in 4% paraformaldehyde (Fisher Scientific) in 1× PBS (Gibco) for 30 minutes. After blocking in 1% BSA solution for 30 minutes, cells were equally divided into 4-well chamber slides which had previously been plated with mitotically inactivated MEFs. Cells were then double stained with one of 14 biotinylated lectins (all lectins obtained from Vector Laboratories; Burlingame, CA; 10 μg/ml) and SSEA 4 (MC 813-70, Developmental Studies Hybridoma Bank [DSHA]; Iowa City;1:100) for 20 minutes at 37°C, followed by 3 washes in PBS. Secondary antibodies included streptavidin conjugated Alexafluor 594 (Molecular Probes; Eugene, OR; 1:250 dilution) and antigoat Mouse IgG conjugated Alexa 488 (Molecular Probes; 1:2000 dilution). During staining procedure, cells were kept at 37°C. Cells were washed three times after secondary antibody incubation for 5 minutes in PBS. Cells were then post-stained with 5 ng/ml DAPI (Sigma) to detect cell nuclei and washed overnight in PBS. Controls of unstained cells were obtained by incubation with secondary antibodies alone. All slides were mounted and visualized using a Nikon TS100 inverted microscope. Individual color channels were captured separately and merged in Adobe Photoshop.
human embryonic stem cells
stage specific embryonic antigen-4
inner cell mass
Fibroblast growth factor-2
mouse embryonic fibroblasts
phosphate buffered saline
fetal bovine serum
bovine serum albumin
- (Con A):
Phaseolus vulgaris erythro-agglutinin
Phaseolus vulgaris leuco-agglutinin
Sambucus nigra agglutinin
Arachis hypogea peanut
Vicia villosa agglutinin
Ricinus communis agglutinin
Wisteria floribunda agglutinin
Ulex europaeus agglutinin
Lotus tetragonolobus lectin
Dolichos biflorus agglutinin
Hippeastrum hybrid lectin
Lycopersicon esculetum tomato
We would like to acknowledge Scott Noggle for magnetic bead sorting protocol; Deb Weiler for culture and maintenance of mouse embryonic fibroblast feeder layers; MaryAnne Della-Fera and Raj Rao for manuscript review and Bresagen, Inc. for supplying the hESC cells.
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